The Value of Homocysteine and Mthfr C677t Polymorphism in the Development of Preeclampsia

Syundyukova E.G., Medvedev B.I., 
Sashenkov S.L., Kvyatkovskaya S.V., 
Dvorchik E.E., Shestakov E.V.

«South-Ural State Medical University», Chelyabinsk, Russian Federation
Clinic of «South-Ural State Medical University», Chelyabinsk, Russian Federation

Abstract. The purpose of the study — to examine the values of homocysteine and MTHFR C677T polymorphism in the development of preeclampsia. In the study, «case¬control» involved 14 women without preeclampsia, 16 — with moderate and 14 to severe preeclampsia; assessed levels of homocysteine and hsСRB, polymorphisms of the gene MTHFR C677T. Found that severe preeclampsia is associated with elevated homocysteine levels (10,48±0,79 mkmol/l) and a high frequency of homozygous mutation MTHFR C6777T (35,7%). The positive correlation of homocysteine with hsCRB (r=0,445, p=0,002), talking about his probable participation in the development of systemic inflammatory response. The positive correlations homocysteine levels (r=0,451, p=0,002) and MTHFR TT (r=0,415, p=0,005) with the frequency and severity of preeclampsia suggests the possibility of using these markers as predictors of preeclampsia .

Key words: pregnancy, preeclampsia, homocysteine, polymorphism of MTHFR C677T.

REFERENCES
1. Bicadze, V.O., Bajmuradova S.M., Talalaeva I.N. Folate¬scarce states and uglinesses of fetus. Zhurnal Rossijskogo obshhestva akusherov-ginekologov — Magazine of the Russian society of obstetricians-gynecologists. 2008. no. 2. pp. 42–48.
2. Je.M. Dzhoboeva, Ju.Je. Dobrohotova. Disfunkcija jendotelija i sistema gemostaza u beremennyh iz grupp vysokogo riska. Sistemnyj podhod k diagnostike i terapii (klinicheskie rekomendacii). [Dysfunction of an endothelium and hemostasis system at pregnant women from groups of high risk. Systems approach to diagnostics and therapy (clinical recommendations)]. Moscow. OOO «Teler» Publ., 2013, 128 p.
3. Makarov O.V., Ozolinja L.A., Shajkova D.A., Kashezheva A.Z.. Pathogenetic aspects of development of obstetric pathology at a gipergomotsisteinemiya. Obstetrics and Gynecology — Akusherstvo i ginekologiya. 2008. no. 4. pp. 3–5.
4. Medvedev B.I., Syundyukova E.G., Sashenkov S.L. Clinical and biochemical predictors for the development of preeclampsia. Obstetrics and Gynecology — Akusherstvo i ginekologiya. 2013. no. 5. pp. 30–35.
5. Medyannikova I.V., Gudinov Zh.V. Spread of genetic polymorphisms associated with gestational thrombohemorrhagic and vascular complications in a cohort of pregnant women from the Russian population. Obstetrics and Gynecology — Akusherstvo i ginekologiya. 2012. no. 4. pp. 10–15.
6. M.A. Repina, G.F. Sumskaja, E.N. Lapina. Nasledstvennye narushenija gemostaza i beremennost'. Metodicheskie rekomendacii. [Heritable violations of a hemostasis and pregnancy. Methodical recommendations]. St. Petersburg. N-L Publ. 2008. 26 с.
7. Sidorova I.S., Nikitina N.A. A current view on the problem of preeclampsia: Arguments and facts. Obstetrics and Gynecology — Akusherstvo i ginekologiya. 2013. no. 5. pp. 10–16.
8. Trifonova E.A., Gabidulina T.V., Agarkova T.A., Gabitova N.A., Stepanov V.A. Homocysteine, MTHFR gene polymorphisms, and pregnancy complications. Obstetrics and Gynecology — Akusherstvo i ginekologiya. 2011. no. 2. pp. 8–15.
9. Chulkov V.S., Vereina N.K., Sinitsyn S.P. Hyperhomocysteinemia, C¬677T Polymorphism of Methylenetetrahydrofolate Reductase (MTHFR) Gene, and Chronic Arterial Hypertension in Pregnant Women. Kardiovaskuljarnaja terapija i profilaktika — Cardiovascular Therapy and Prevention. 2012. no. 1. pp. 41–44.
10. Chulkov V.S., Vereina N.K., Sinitsyn S.P. Assessment of the Relationship of Genetic and Acquired Factors with Pregnancy Complications in Women with Different Forms of Hypertension. Sibirskij medicinskij zhurnal — Siberian Jornal of Medicine. 2013. no. 3. pp. 76–78.
11. Devaraj, S. The evolving role of C-reactive protein in atherothrombosis. S. Devaraj. Clin Chem. 2009. vol. 55. no. 2. pp. 229–238.
12. Hogeveen, M. Maternal homocysteine and small-for-offspring: systematic review and meta¬analisis. M. Hogeveen, M. Hogeveen, H.J. Blom, M. den Heijer. Am. J. Clin. Nutr. 2012. vol. 95. no. 1. pp. 130–136.
13. Jääskeläinen, E. MTHFR C677T polymorphism is not associated with placental abruption or preeclampsia in Finnish women. E.Jääskeläinen, L. Keski-Nisula, S. Toivonen, E.L. Romppanen, S. Helisalmi, K. Punnonen, S. Heinonen//Hypertens. Pregnancy. 2006. vol. 25. no. 2. pp. 73–80.
14. Murphy, M.M. Homocysteine in pregnancy. M.M. Murphy, J.D. Fernandez-Ballart. Adv. Clin. Chem. 2011. no. 53. pp. 105–137.
15. World Health Organization. WHO recommendations for prevention and treatment of pre¬eclampsia and eclampsia. Geneva. 2011.

Authors
Syundyukova Elena G. 
e¬mail: seg269@mail.ru
«South¬Ural State Medical University», Chelyabinsk, Russian Federation
Associate professor of chair of Obstetrics and Gynecology, candidate of medical sciences

Medvedev Boris I.
«South-Ural State Medical University», Chelyabinsk, Russian Federation
Associate professor of chair of Obstetrics and Gynecology, candidate of medical sciences;
Professor of chair of Obstetrics and Gynecology, doctor of medical sciences

Sashenkov Sergey L.
«South-Ural State Medical University», Chelyabinsk, Russian Federation
Professor of chair of Normal Physiology, doctor of medical sciences

Kvyatkovskaya Svetlana V.
Clinic of «South-Ural State Medical University», Chelyabinsk, Russian Federation
Head of immunological laboratory of, candidate of medical sciences

Dvorchik Elena E.
Clinic of «South-Ural State Medical University», Chelyabinsk, Russian Federation
Doctor immunology laboratory of, candidate of medical sciences

Shestakova Elena V.
Clinic of «South-Ural State Medical University», Chelyabinsk, Russian Federation
candidate of medical sciences

454091, Russian Federation, Chelyabinsk, Vorovsky Str. 64.
e-mail: seg269@mail.ru