The Influence of Chitin on the Mitotic Cycle of Fibroblasts in Culture

Shamshurina E.O., Yushkov B.G., Sazonov S.V.

Institute of Immunology and physiology of UB RAS;
GBOU HPE Ural State Medical University of Health Ministry of Russia;
GBUZ SO Institute for Medical Cell Technologies

Abstract. The aim of our research was the influence of chitin on the phases of fibroblast`s mitotic cycle. The results of the research showed, that chitin would reduce the phases of the fibroblast’s mitosis and the time of generation of cells in culture. But under the affect of the chitin cells are held-up in the synthetic period of the fibroblast’s cycle. The change of the quality of the cell’s cycle leads to existence polynuclear cells in the fibroblast’s culture.
Thus, chitin change the ability for the cells pass the phases of mitotic cycle and the character of the fibroblast’s culture.

Key words: fibroblasts, cell culture, cell cycle, glycosaminoglycan, chitin, proliferation.

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Authors
Shamshurina Elena O. E-mail: Imct@celltechnologis.ru
GBUZ SO Institute for Medical Cell Technologies
Russian Federation, 620 036, Ekaterinburg, Soboleva, 25
Yushkov Boris G. 
Institute of Immunology and physiology of UB RAS
Russian Federation, Ekaterinburg, UL. Pervomaiskaya, 109
Sazonov Sergey V. E-mail: Prof-SSazonov@yandex.ru
GBOU HPE Ural State Medical University of Health Ministry of Russia
Russian Federation, Ekaterinburg 620028, Repina,3
GBUZ SO Institute for Medical Cell Technologies, Ekaterinburg, Russian Federation, 620 036, Ekaterinburg, Soboleva, 25