Study of the Cell State at the Cytomegalovirus Infection by Means of CDS Quantum Dots
Rempel S.V., Alexandrova N.N., Poryvaeva A.P., Rempel А.А.
Institute of Solid State Chemistry, Ural Division of the Russian Academy of Sciences, Yekaterinburg, Russian Federation;
FSIS “Ekaterinburg Research Institute of Viral Infections” Russias Inspectorate for Concumer Right Protection and Well-Being, Yekaterinburg, Russian Federation;
Ural Federal University named after the First President of Russia B.N. Yeltsin, Yekaterinburg, Russian Federation
Abstract. The possibility of visualization of cell structures changes on model of a productiveCytomegalovirusinfection by means of the colloidal quantum dots (QD — semiconductor nanocrystals of cadmium sulfide CdS) was studied. The state of cell of light embryo of the human (LECh-3), infected by CMV (AD169 strain) was observed within 72 hours. Registration of fluorescence was carried out on an optical microscope of Leica 2500M DM. Changing of cell up to full destruction of a monolayer was registered. The possibility of using of CdS QD as fluorescent labels of new generation is shown.
This work was financially supported by project of Russian Fond of Basic Research No. 13-04-96069.
Key words: сytomegalovirusinfection, cell culture, quantum dots
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Authors
Rempel Svetlana V.
Alexandrova Nina N.
Poryvaeva Antonina P.
Rempel A.A.
Institute of Solid State Chemistry, Ural Division of the Russian Academy of Sciences
620990, Yekaterinburg, Russian Federation, Pervomaiskaya, 91, Institute of Solid State Chemistry.
Ural Federal University named after the First President of Russia B.N. Yeltsin,
620002, Yekaterinburg, Russian Federation Mira str. 19
FSIS “Ekaterinburg Research Institute of Viral Infections” Russias Inspectorate for Concumer Right Protection and Well-Being.
23, Letnyaya St., FSIS “Ekaterinburg Research Institute of Viral Infections”, Yekaterinburg, Russian Federation