Morphokinetic Characteristics and Implantation Potential of Human Embryos Obtained During Art Cycles

Pravdyuk A. I., Gryshchenko M. G., Parashchuk V. Yu.

Academician V. I. Gryshchenko Clinic for Reproductive Medicine, Kharkov, Ukraine

Abstract.
In this paper morphokinetics of embryos with known implantation was analyzed. Contrary to expectations, a significant number of the embryos from this group had the worst implantation prognosis when assessed with morphokinetics criteria classification only. Thus, applied classification did not accurately predict implantation of embryos in terms of our laboratory and clinical conditions. Probably, the predictive value of this classification can be improved by integrating data on the impact of various factors on the dynamics of the development of embryos in assisted reproduction cycles.
Some factors were evaluated for their impact on embryo morphokinetics during ART cycles. We found that application of different controlled ovarian stimulation protocols and gonadotropin dose is accompanied by changes in morphokinetic characteristic of embryos. However, both male and female age, the number of oocytes and number of normal forms of sperm in the ejaculate did not affect the kinetic parameters of embryo development. Further research is needed to identify all the spectrum of factors, which can affect the rate of embryo development.
Keywords:
COS protocol, embryo, FSH, implantation, IVF, morphokinetics, time-lapse monitoring

REFERENCES

1. Sfontouris I. A. Non-invasive metabolomic analysis using a commercial nir instrument for embryo selection. I. A. Sfontouris, G. T. Lainas, D. Sakkas [et al.]. Journal of human reproductive sciences. 2013. Vol. 6, No. 2. pp. 133–139.

2. Reich A. The transcriptome of a human polar body accurately reflects its sibling oocyte / A. Reich, P. Klatsky, S. Carson, G. Wessel. The Journal of biological chemistry. 2011. Vol. 286, No. 47. pp. 40743–40749.
3. Rubio C. Use of array comparative genomic hybridization (array-cgh) for embryo assessment: clinical results. C. Rubio, L. Rodrigo, P. Mir [et al.] Fertility and sterility. 2013. Vol. 99, No. 4. pp. 1044–1048.
4. Herrero J. Selection of high potential embryos using timelapse imaging: the era of morphokinetics. Herrero, M. Meseguer. Fertility and sterility. 2013. Vol. 99, No. 4. pp. 1030–1034.

5. Chen A. A. Biomarkers identified with time-lapse imaging: discovery, validation, and practical application. A. A. Chen, L. Tan, V. Suraj [et al.] Fertility and sterility. 2013. Vol. 99, No. 4. pp. 1035–1043.
6. Meseguer M. The use of morphokinetics as a predictor of embryo implantation. M. Meseguer, J. Herrero, A. Tejera [et al.]. Human reproduction (Oxford, England). 2011. Vol. 26, No. 10. pp. 2658–2671.
7. Wissing M. L. Impact of pcos on early embryo cleavage kinetics. M. L. Wissing, M. R. Bjerge, A. I. G. Olesen [et al.] Reproductive biomedicine online. 2013.
8. Ciray H. N. Time-lapse evaluation of human embryo development in single versus sequential culture media-a sibling oocyte study. H. N. Ciray, T. Aksoy, C. Goktas [et al.] Journal of assisted reproduction and genetics. 2012. Vol. 29, No. 9. pp. 891–900.
9. Zaninovic N. Impact of oxygen concentration on embryo development, embryo morphology and morphokinetics. N. Zaninovic, J. Goldschlag, H. Yin [et al.] Fertility and Sterility. 2013. Vol. 100, No. 3. P. S240.
10. Fréour T. Comparison of embryo morphokinetics after in vitro fertilization-intracytoplasmic sperm injection in smoking and nonsmoking women. T. Fréour, L. Dessolle, J. Lammers [et al.] Fertility and sterility. 2013. Vol. 99, No. 7. pp. 1944–1950.
11. Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology The istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology. Human reproduction (Oxford, England). 2011. Vol. 26, No. 6. pp. 1270–1283.
12. Yan J. Effect of maternal age on the outcomes of in vitro fertilization and embryo transfer (ivf-et). J. Yan, K. Wu, R. Tang [et al.] Science China. Life sciences. 2012. Vol. 55, No. 8. pp. 694–698.
13. Preutthipan S. Effect of maternal age on clinical outcome in women undergoing in vitro fertilization and embryo transfer (ivf-et). S. Preutthipan, N. Amso, P. Curtis, R. W. Shaw. Journal of the Medical Association of Thailand = Chotmaihet thangphaet. 1996. Vol. 79, № 6. pp. 347–352.
14. Eichenlaub-Ritter U. Genetics of oocyte ageing. U. Eichenlaub-Ritter. Maturitas. 1998. Vol. 30, No. 2. pp. 143–169.
15. Fragouli E. Chromosome abnormalities in the human oocyte. E. Fragouli, D. Wells, J. D. A. Delhanty. Cytogenetic and genome research. 2011. Vol. 133, No. 2–4. pp. 107–118.
16. Eichenlaub-Ritter U. Spindles, mitochondria and redox potential in ageing oocytes. U. Eichenlaub-Ritter, E. Vogt, H. Yin, R. Gosden. Reproductive biomedicine online. 2004. Vol. 8, No. 1. pp. 45–58.
17. Frattarelli J. L. Male age negatively impacts embryo development and reproductive outcome in donor oocyte assisted reproductive technology cycles. J. L. Frattarelli, K. A. Miller, B. T. Miller [et al.] Fertility and sterility. 2008. Vol. 90, No. 1. pp. 97–103.
18. Klonoff-Cohen H. S. The effect of advancing paternal age on pregnancy and live birth rates in couples undergoing in vitro fertilization or gamete intrafallopian transfer. H. S. Klonoff-Cohen, L. Natarajan. American journal of obstetrics and gynecology. 2004. Vol. 191, No. 2. pp. 507–514.
19. Bellver J. Influence of paternal age on assisted reproduction outcome. J. Bellver, N. Garrido, J. Remohí [et al.] Reproductive biomedicine online. 2008. Vol. 17, No. 5. pp. 595–604.
20. Muñoz M. Dose of recombinant fsh and oestradiol concentration on day of hcg affect embryo development kinetics. M. Muñoz, M. Cruz, P. Humaidan [et al.] Reproductive biomedicine online. 2012. Vol. 25, No. 4. pp. 382–389.
21. Yilmaz N. Is there a detrimental effect of higher gonadotrophin dose on clinical pregnancy rate in normoresponders undergoing art with long protocol? N. Yilmaz, S. Yilmaz, H. Inal [et al.] Archives of gynecology and obstetrics. 2013. Vol. 287, No. 5. pp. 1039–1044.
22. Lammers J. Effect of female underweight on embryo morphokinetic using time-lapse. J. Lammers, T. Freour, C. Splingart, P. Barriere. Human Reproduction. 2013. No. 28 (Suppl. 1): P-085.
23. Navarro-Costa P. Incorrect dna methylation of the dazl promoter cpg island associates with defective human sperm. P. Navarro-Costa, P. Nogueira, M. Carvalho [et al.] Human reproduction (Oxford, England). 2010. Vol. 25, № 10. pp. 2647–2654.
24. Ugajin T. The shape of the sperm midpiece in intracytoplasmic morphologically selected sperm injection relates sperm centrosomal function. T. Ugajin, Y. Terada, H. Hasegawa [et al.] Journal of Assisted Reproduction and Genetics. 2010. Vol. 27, No. 2–3. pp. 75–81.

25. Maettner R. Quality of human spermatozoa: relationship between high-magnification sperm morphology and dna integrity. R. Maettner, K. Sterzik, V. Isachenko [et al.] Andrologia. 2013.

26. Sunkara S. K. Association between the number of eggs and live birth in ivf treatment: an analysis of 400 135 treatment cycles. S. K. Sunkara, V. Rittenberg, N. Raine-Fenning [et al.] Human reproduction (Oxford, England). 2011. Vol. 26, No. 7. pp. 1768–1774.
27. Kwee J. Ovarian volume and antral follicle count for the prediction of low and hyper responders with in vitro fertilization. J. Kwee, M. E. Elting, R. Schats [et al.] Reproductive biology and endocrinology: RB&E. 2007. Vol. 5. P. 9.

Authors
Gryshchenko Mykola G.
Academician V. I. Gryshchenko Clinic for Reproductive Medicine
DM, professor, director
25 Karl Marx str. Kharkov, Ukraine, 61052
e-mail: gryshchenko@implant-ivf.com

Pravdyuk Alexey I.
PhD, embryologist
e-mail: pravduke@yandex.ru

Parashchyuk Valentin Yu.
PhD, physician
e-mail: parashchuk@implant-ivf.com