Analysis of Biofilm-Forming Ability of Clinical Strains of Coagulase-Negative Staphylococci
Poljudova T. V., Lemkina L. M., Korobov V. P.
Federal State Budget Establishment of Science Institute of Ecology and Genetics of Microorganisms, Ural Branch of Russian Academy of Sciences, Perm, Russian Federaition
Abstract.
The ability of clinical strains of coagulase-negative staphylococci to form biofilm was studied. The bacterial growth on Congo red agar, the ability to the hemagglutination, the cell surface hydrophobicity were characterized. The biofilm intensity depends on the nutrient medium and the Na+ ions presence. The peptide antibacterial compounds such as lysostaphin and cationic low molecular weight peptide warnerin, also the hydrolytic enzyme proteinase K destroyed the mature biofilm of coagulase-negative staphylococci.
Keywords:
coagulase-negative staphylococci, biofilm, cationic peptides
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Authors
Poljudova Tat'jana V.
poludova@iegm.ru
Candidate of Biological Sciences, Senior Researcher
Lemkina Larisa M.
l.lemkina@iegm.ru
Candidate of Medical Sciences, Senior Researcher
Korobov Vladimir P.
korobov@iegm.ru
Candidate of Medical Sciences, Senior Researcher, Head of the Laboratory of Biochemistry of Microorganisms
Institute of Ecology and Genetics of Microorganisms Ural Branch of the Russian Academy of Sciences
614081Perm, Goleva str. 13, Russian Federation