Apoptosis of monocytes in the conditions of the general cooling at persons with background monocytosis

DOI: 10.22138/2500-0918-2019-16-2-202-208

O.A. Stavinskaya

Institute of Environmental Physiology of N. Laverov Federal Center for Integrated Arctic Research Russian Academy of Sciences, Arkhangelsk, Russian Federation

Abstract. Aim — study dynamics of programmed death of monocytes after short-term cooling at persons with a background monocytosis. Materials and methods. Examination of 56 people aged from 20 up to 50 years before short-term general cooling (5 minutes in cold camera at –25 °C). At the same time the rules of biomedical ethics were followed. The leukogramma of peripheral blood was defined on the hematologic analyser Sysmex XS-500i (Japan). Programmed death of monocytes of peripheral blood was estimated on the maintenance of polymorfonucleus cells as a part of the monocytogramma on a microscope Vision MT5300L (Japan). By method of the enzyme immunoassay on the automatic analyser Evolis (Bio-RAD, France) serum concentration of TNF-α, IL-1β, IL-6, IL-10 (Bender MedSystems, Austria) revealed. Results and discussion. The analysis of results of a research was carried out, comparing levels of responses depending on the content in blood before stay in cold camera of the circulating monocytes (<0,6×109 кл/л, n=35 и ≥0,9×109 кл/л, n=21, p<0,001). It is established that at the normal maintenance of monocytes decrease in maintenance of mature T-cells, phagocytic activity and also TNFα is observed; against the background of a monocytosis concentration mature, activated and differentiated are higher than T-cells, cytokine reaction is limited to increase in maintenance of IL-1β. Cold factor increases apoptosis of monocytes in cases of a monocytosis; at the normal maintenance of monocytes in blood of statistically significant increase in activity of apoptosis of monocytes it is not set. Conclusion. Monocytosis is provided with secreted monocytes with low phagocytic activity, is associated with increase in content in blood of the mature and activated T- and B-lymphocytes, IL-6 and is regulated by increase in activity of apoptosis of these cells.

Keyword: monocytosis, apoptosis of monocytes, general cooling, phenotypes, cytokines, almost healthy people

Received 15.04.2019.

For citation:
Stavinskaya O.A. Apoptosis of monocytes in the conditions of the general cooling at persons with background monocytosis. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2019, Vol. 16, no. 2, pp. 202–208. DOI: 10.22138/2500-0918-2019-16-2-202-208 (In Russ)

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Аuthor
Ol'ga A. Stavinskaya
Institute of Environmental Physiology of N. Laverov Federal Center for Integrated Arctic Research Russian Academy of Sciences
Cand. Sci. (Biology), Senior scientific researcher
Lomonosova pr. 249 Arhangel'sk Russian Federation 163000
ifpa-olga@mail.ru

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