BIOELECTRICAL IMPEDANCE OF THE LUNG IN RATS UNDER CHRONIC EXPOSURE TO DOXORUBICIN
DOI: 10.22138/2500-0918-2022-19-4-386-392
N. L. Kolomeyets, O. V. Syslonova, S. L. Smirnova, I. M. Roshchevskaya
Federal Research Centre “Komi Science Centre of the Ural Branch
of the Russian Academy of Sciences”, Syktyvkar, Komi Republic, Russian Federation
Abstract. Changes in lung bioelectric impedance were revealed in male Wistar rats exposed chronically to doxorubicin. Bioimpedance measurements were performed in rats two months after the first injection of doxorubicin (a cumulative dose of 10 mg/kg divided into 4 injections for 4 weeks) or saline. Bioimpedance was analysed at multiple frequencies (10, 30, 70 and 150 kHz) of electric current. Electrodes were placed on the surface of the lung. Significantly lower absolute values of the reactance and phase angle of bioelectrical impedance of the myocardium, liver (noted by us earlier) and lung in rats treated with doxorubicin compared with control animals indicated toxic damage to organs. Significantly higher amplitude (at a frequency of 70 kHz) of bioelectrical impedance of the rat’s lung may be associated with emphysematous changes and inflammation in the lung in the group with chronic exposure to doxorubicin compared to the rats in the control group.
Keywords: doxorubicin, bioelectrical impedance of the lung
There is no conflict of interest.
Contact details of the corresponding author:
Nataliya L. Kolomeyets
smirnova.sl@mail.ru
Received 14.10.2022
For citation:
Kolomeyets N.L., Syslonova O.V., Smirnova S.L., Roshchevskaya I.M. Bioelectrical impedance of the lung in rats under chronic exposure to doxorubicin. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2022, Vol. 19, no. 4, pp. 386–392. DOI: 10.22138/2500-0918-2022-19-4-386-392 (In Russ)
REFERENCES
1. Gendlin G.E., Emelina E.I., Nikitin I.G., Vasyuk Yu.A. Modern view on cardiotoxicity of chemotherapeutics in oncology including anthracyclines. Russian Journal of Cardiology. 2017, (3): 145-154, doi: 10.15829/1560-4071-2017-3-145-154. (In Russ.)
2. Wallace K.B., Sardão V.A., Oliveira P.J. Mitochondrial Determinants of Doxorubicin-Induced Cardiomyopathy. Circulation Research, 2020, 126: 926–941. doi: 10.1161/circresaha.119.314681
3. Irfan O., Gilani J.A., Irshad A., Irfan B., Khan J.A. Pharmacological Threat to Lungs: A Case Series and Literature Review. Cureus. 2017, 9(5): e1232. doi: 10.7759/cureus.1232.
4. Schwaiblmair M., Behr W., Haeckel T., Märkl B., Foerg W., Berghaus T. Drug induced interstitial lung disease. Open Respir. Med. J. 2012, 6: 63-74. doi: 10.2174/1874306401206010063.
5. Armenian S., Bhatia S. Predicting and Preventing Anthracycline-Related Cardiotoxicity. Am. Soc. Clin. Oncol. Educ. Book. 2018, 38: 3-12. doi: 10.1200/EDBK_100015.
6. Pérez García I., González Islas D., Orea Tejeda A., Sánchez Santillan R., Verdeja Vendrell L., Hernández Centeno R., et al. Bioelectrical impedance vector analysis in COPD patients with right heart failure. European Respiratory Journal 2020; 56: Suppl. 64. 1258. doi: 10.1183/13993003.congress-2020.1258
7. Zink M.D., König F., Weyer S., Willmes K., Leonhardt S., Marx N. et al. Segmental Bioelectrical Impedance Spectroscopy to Monitor Fluid Status in Heart Failure. Sci. Rep. 2020, 10: 3577. doi: 10.1038/s41598-020-60358-y.
8. Orea-Tejeda A., Gómez-Martínez M., González-Islas D., Flores-Cisneros L., Keirns-Davis C., Sánchez-Santillán R., Pérez-García I., Martínez-Luna N., Robles-Hernández R., Sánchez-Moreno C., Orozco-Gutíerrez J.J. The impact of hydration status and fluid distribution on pulmonary function in COPD patients. Sci. Rep. 2022, 12(1):1216. doi: 10.1038/s41598-022-05192-0.
9. Injac R., Radic N., Govedarica B., Perse M., Cerar A., Djordjevic A., et al. Acute doxorubicin pulmotoxicity in rats with malignant neoplasm is effectively treated with fullerenol C60(OH)24 through inhibition of oxidative stress. Pharmacol. Rep. 2009, 61(2): 335-42. doi: 10.1016/s1734-1140(09)70041-6
10. Machado N.G., Baldeiras I., Pereira G.C., Pereira S.P., Oliveira P.J. Sub-chronic administration of doxorubicin to Wistar rats results in oxidative stress and unaltered apoptotic signaling in the lung. Chemico-Biological Interactions. 2010, 188(3): 478-486. doi: 10.1016/j.cbi.2010.09.027.
11. Take G., Yamaç D., Ozoğul C., Erdogan D. Ultrastructural Damage in Lung Tissues in Rats Treated with Doxorubicin and Paclitaxel. Adv. Ther. 2008, 25(2): 115-122. doi: 10.1007/s12325-008-0015-0.
12. Minchin R.F., Johnston M.R., Schuller H.M., Aiken M.A., Boyd M.R. Pulmonary toxicity of doxorubicin administered by in situ isolated lung perfusion in dogs. Cancer. 1988, 61(7):1320-5. doi: 10.1002/1097-0142(19880401)61:73.0.co;2-j
13. Kolomeyets N.L., Syslonova O.V., Smirnova S.L., Gulyaeva A.S., Roshchevskaya I.M. Bioelectrical impedance parameters of the lung in female Wistar rats with experimental pulmonary hypertension. Russian Journal of Physiology. 2019, 105 (8): 1041-1053. doi: 10.1134/S0869813919080089. (in Russian)
14. Kolomeyets N.L., Syslonova O.V., Smirnova S.L., Peshkin E.A., Roshchevskaya I.M. Bioelectrical Impedance of the Rat Myocardium and Liver under Chronic Exposure to Doxorubicin. J Evol. Biochem. Phys. 2021, 57: 1351–1362. doi: 10.1134/S0022093021060144. (in Russian)
15. Nopp P., Rapp E., Pfutzner H., Nakesch H., Ruhsam C. Dielectric properties of lung tissue as a function of air content. Phys. Med. Biol. 1993, 38:699-716. doi: 10.1088/0031-9155/38/6/005.
Authors
Nataliya L. Kolomeyets
PhD, Researcher
kolomeec@frc.komisc.
Olga V. Suslonova
Researcher
evgeniu2006@inbox.ru
Svetlana L. Smirnova
PhD, head of the Department of Comparative Cardiology
smirnova.sl@mail.ru
Irina M. Roshchevskaya
MD, Corresponding Member of the Russian Academy of Sciences, Chief Researcher
compcard@mail.ru
Department of Comparative Cardiology, Federal Research Centre “Komi Science Centre of the Ural Branch of the Russian Academy of Sciences”
24 Kommunisticheskaya str., Syktyvkar Komi Republic Russian Federation 167982