BIOMARKERS OF THE COMMON PATHOGENETIC LINK OF IMMUNE-MEDIATED SKIN DISEASES
DOI: 10.22138/2500-0918-2024-21-4-377-389
D.S. Zagreshenko¹, Ya.V. Kukharev², A.V. Klimov², V.V. Klimov²,
M.M. Rahmanova³, M.I. Musina⁴, A.N. Shkatova⁴, M.I. Slezkin⁵, S.A. Hardikova², V.V. Pestova⁴, I.S. Yakovenko³, I.Yu. Barkova³, A.R. Vagul⁴
¹Novokuznetsk State Institute for Postgraduate Medical Education,
Novokuznetsk, Russian Federation;
²Siberian State Medical University, Tomsk, Russian Federation;
³Novokuznetsk City Clinical Hospital No.1 named after G.P.Kurbatov,
Novokuznetsk, Russian Federation;
⁴Interuniversity polyclinic, Tomsk, Russian Federation;
⁵Immunopathology Center Ltd, Tomsk, Russian Federation
Resume. Currently, not all skin diseases have a sufficiently well-studied etiology and pathogenesis. Objective: to determine the concentration of adrenocorticotropic hormone, zinc and cytokines (IL-1β, IL-18, IL-6, TNF-α) in the cell-free fraction of the exudate of the «skin window» in scleroderma, psoriasis, atopic dermatitis and chronic urticaria. Materials and methods. 42 patients with mild and moderate atopic dermatitis, 41 with limited scleroderma, 44 patients with chronic spontaneous urticaria and 40 patients with plaque psoriasis aged 18 to 50 years were examined. The control group consisted of 25 donors aged 20 to 35 years. The material for the study of adrenocorticotropic hormone, zinc and cytokines at the local level (in the skin) was a cell-free fraction of the «skin window» exudate obtained from a 1 ml chamber installed on a scarified area of the skin. The tank was pre-filled with a sterile 0.9% sodium chloride solution. Results. In patients with all studied immuno-mediated skin diseases of different etiology and pathogenesis, the same type of changes were revealed in the cell-free exudate of the cutaneous window. Conclusion. The association of the presence of a pathological process with an increase in the local level of proinflammatory cytokines and zinc, as well as with a decrease in the concentration of adrenocorticotropic hormone lower than in the donor group, is shown.
Keywords: cytokines, “skin window”, comorbidity, atopy, adrenocorticotropic hormone, zinc, inflammasoma, dermatology, atopic dermatitis, allergic rhinitis
There is no conflict of interest.
Contact details of the corresponding author:
Yaroslav V. Kukharev
kukharev78@mail.ru
Received: 01.09.2024
For citation: Zagreshenko D.S., Kukharev Ya.V., Klimov A.V., Klimov V.V., Rahmanova M.M., Musina M.I., Shkatova A.N., Slezkin M.I., Hardikova S.A., Pestova V.V., Yakovenko I.S., Barkova I.Yu., Vagul A.R. Biomarkers of the common pathogenetic link of immune-mediated skin diseases. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2024, Vol. 21, no. 4, pp. 377–389. DOI: 10.22138/2500-0918-2024-21-4-377-389 (In Russ)
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Authors
Denis S. Zagreshenko
Novokuznetsk State Institute for Postgraduate Medical Education – Branch Campus of the FSBEI FPE RMACPE MOH, Novokuznetsk
PhD (Medicine), Associate Professor of the Department of Clinical Laboratory Diagnostics
Novokuznetsk, Russian Federation
zagreshenko@rambler.ru
Yaroslav V. Kukharev
Siberian State Medical University (SibSMU)
PhD (Medicine), Immunology&AllergyDept Assistant Professor
Tomsk, Russian Federation
kukharev78@mail.ru
Andrew V. Klimov
Siberian State Medical University (SibSMU)
PhD (Medicine), Immunology&AllergyDept Associate Professor
Tomsk, Russian Federation
klimov.lor@mail.ru
Vladimir V. Klimov
Siberian State Medical University (SibSMU)
PhD (Medicine), MD, Immunology & Allergy Dept Head
Tomsk, Russian Federation
vlklimov54@gmail.com
Malika M. Rahmanova
Novokuznetsk City Clinical Hospital No.1 named after G.P.Kurbatov
Generalist
Novokuznetsk, Russian Federation
rahmanova.malika93@mail.ru
Marina I. Musina
Interuniversity polyclinic
Head
Tomsk, Russian Federation
mvpol@tomsk.gov70.ru
Alina N. Shkatova
Interuniversity polyclinic
PhD (Medicine), Allergy Unit Head
Tomsk, Russian Federation
alinashkatik@gmail.com
Michael I. Slezkin
Immunopathology Center Ltd
Biochemist
Tomsk, Russian Federation
smimmm5@gmail.com
Svetlana A. Hardikova
Siberian State Medical University (SibSMU)
PhD (Medicine), MD, Dermatovenerology & Cosmetology Dept Head
Tomsk, Russian Federation
khardikova.s.a@mail.ru
Vera V. Pestova
Interuniversity polyclinic
Vice-head
Tomsk, Russian Federation
mvpol@tomsk.gov70.ru
Irina S. Yakovenko
Novokuznetsk City Clinical Hospital No.1 named after G.P. Kurbatov
Generalist
Novokuznetsk, Russian Federation
andrianova-inna2@mail.ru
Irina Yu. Barkova
Novokuznetsk City Clinical Hospital No.1 named after G.P. Kurbatov
Generalist
Novokuznetsk, Russian Federation
nuisible3@mail.ru
Alla R. Vagul
Interuniversity polyclinic
Clinical diagnostic laboratory, head
Tomsk, Russian Federation
mvpol@tomsk.gov70.ru