Laboratory Animals Cytokine Status under Helminthosis and its Treatment
E.A. Grishina
DOI: 10.22138/2500-0918-2017-14-4-332-34
Russian Medical Academy of Continuous Professional Education, Moscow, Russian Federation
Abstract. The aim of the present work was to investigate a temporal profile of pro- and anti-inflammatory cytokines (IL-l and IL-4) and their ratio (IL-l/ IL-4) in the serum of mice after experimental infection with Syphacia obvelata or Trichuris trichiura within the first 3 weeks of infestation as well as on the background of etiotropic therapy with levamisole alone and in combination with roncoleukin. It was found that in the dynamics of the experimental infestations (Syphacia obvelata or Trichuris trichiura) within the period from 3d to 21st day there was a significant tendency of increasing IL-1 level, while the growth of IL-4 concentration was less pronounced. The IL-1/IL-4 ratio steadily increased without pronounced symptoms of inflammation. In the group of animals infected with T. trichiura and receiving mono-therapy with levamisole cytokine concentration decreased gradually to the control values, while in the group infected with S. obvelata this indicator maintained at the same level. Thus, the capacity of levamisole, which has anthelmintic and immunomodulating properties, to normalize cytokine status, which significantly changes with the development of invasions, has been confirmed. Roncoleukin comprises pro-inflammatory cytokine IL-2. So the use of complex therapy which combined both levamisole and roncoleukin normalized IL-1/IL-4 ratio holding the balance between pro- and anti-inflammatory responses to a constant level during both invasions.This study confirmed that determining the concentration of the pro-inflammatory cytokine IL-1 and anti-inflammatory cytokine IL-4 as well as their ratio can be exploited to dynamically control the invasive process and the effectiveness of the therapy. Monotherapy with levamisole results in an insignificant correction of the cytokine status, and the use of complex therapy with the immunomodulator allows to keep the balance between pro- and anti-inflammatory reactions at a constant level.
Keywords: helminthiases, immunopathology, cytokines, levamisole, roncoleukin
Received 06.09.2017
For citation:
Grishina E.A. Laboratory animals cytokine status under helminthosis and its treatment. Vestn. Ural. Med. Akad. Nauki. Journal of Ural Medical Academic Science. 2017, Vol. 14, no. 4, pp. 332–340. DOI: 10.22138/2500-0918-2017-14-4-332-340 (In Russ)
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Autor
Elena A. Grishina
Russian Medical Academy of Continuous Professional Education
Head of Department of Molecular Biology of research Сenter, Cand.Sci (Biol), Associate professor
2/1-1, Barricadnaya ul., Moscow, Russian Federation, 125993
gelana2010@yandex.ru