THE STATE OF MICROCIRCULATION, THE HEMOSTASIS SYSTEM, AND LEVEL OF PROTEINS IRISIN AND CCL11 IN YOUNG AND OLD RATS

DOI: 10.22138/2500-0918-2021-18-3-197-208

I.N. Manaeva, Yu.A. Bondarchuk, I.I. Shakhmatov,

S.V. Moskalenko, I.P. Bobrov, P.S. Marshalkina, D.A. Prokopets

Federal State Budgetary Educational Institution of Higher Education «Altai State Medical University» of the Ministry of Health of Russia, Barnaul, Russian Federation

Abstract. A significant role in the development of cardiovascular pathology in old age belongs to age-related changes in the microvasculature and the hemostatic system. The development of age-related diseases is influenced by both the level and the ratio of the proteins «youth» (irisin) and «old age» (CCL11). To carry out effective prevention of the occurrence of these diseases, it is necessary to study the characteristics of the functioning of an aging organism. The aim is to assess the state of the microvasculature, the hemostasis system, the level of the proteins irisin and the chemokine CCL11 in young and old rats. Young and old male rats (28 individuals) of the Wistar line were used in the experiment. The state of the microvasculature, the hemostasis system of animals, the level of the proteins irisin and CCL11 were studied. In old rats, compared with young animals, at the microcirculatory level, spasm of arterioles was observed on the background of a decrease in nutritional blood flow. An increase in the aggregation activity of platelets in aged animals was accompanied by hypercoagulation, recorded using clotting and global techniques, as well as a decrease in the anticoagulant activity of blood plasma and activation of fibrinolysis. In addition, in older animals, a decrease in the level of the irisin protein was recorded, while the chemokine CCL11 increased. So, with increasing age, there is a significant deterioration in the state of the microvasculature, hypercoagulative shifts in the hemostasis system and unfavorable changes in the level and ratio of proteins of «youth» and «old age».

Keywords: microvasculature, hemostasis system, irisin, CCL11, young rats, old rats

There is no conflict of interest.
Contact details of the corresponding author:
Irina N. Manaeva
krinitsina.irina@mail.ru
Received 21.06.2021
For citation:
Manaeva I.N., Bondarchuk Yu.A., Shakhmatov I.I., Moskalenko S.V., Bobrov I.P., Marshalkina P.S., Prokopets D.A. The state of microcirculation, the hemostasis system, and level of proteins irisin and CCL11 in young and old rats. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2021, Vol. 18, no. 3, pp. 197–208. DOI: 10.22138/2500-0918-2021-18-3-197-208 (In Russ)

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Authors
Irina N. Manaeva
Assistant at the Department of Normal Physiology
krinitsina.irina@mail.ru

Yulia A. Bondarchuk
Candidate of Medical Sciences, Associate Professor of the Department of Normal Physiology
bondarchuk2606@yandex.ru

Igor I. Shakhmatov
Doctor of Medical Sciences, Professor, Head of the Department of Normal Physiology
iish59@yandex.ru

Svetlana V. Moskalenko
Candidate of Medical Sciences, Assistant of the Department of Normal Physiology
sunrisemsv@gmail.com

Igor P. Bobrov
Doctor of Medical Sciences, Professor of the Department of Forensic Medicine named after Professor V.N. Kryukov and pathological anatomy with APE course.
ig.bobrov2010@yandex.ru

Polina S. Marshalkina
5th year student of the Institute of Clinical Medicine
marshalkina.polina@mail.ru

Daria A. Prokopets
5th year student of the Institute of Clinical Medicine
darya_prokopets@mail.ru

Federal State Budgetary Educational Institution of Higher Education «Altai State Medical University» of the Ministry of Health of Russia
40 Lenin Avenue Barnaul Altai Territory Russian Federation 656038

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)