RESPONSE OF THE HEMOSTATIC SYSTEM TO TO REPEATED CYCLES OF HYPERCAPNIC HYPOXIC TRAINING; EXPOSURE TO HYPERCAPNIC HYPOXIA COMBINED WITH MEXIDOL; AND ISOLATED TREATMENT WITH MEXIDOL
УДК 612.22615.23
DOI: 10.22138/2500-0918-2019-16-3-330-341
S.V. Moskalenko, I.I. Shakhmatov, I.V. Kovalev, Yu.A. Bondarchuk, O.V. Alekseeva, O.M. Ulitina
Altai State Medical University, Barnaul, Russian Federation;
State Scientific-Research Institute of Physiology and Basic Medicine, Novosibirsk, Russian Federation;
Siberian State Medical University, Tomsk, Russian Federation
Abstract. Study objectives: The study was conducted to assess the reaction of the hemostatic system to repeated cycles of hypercapnic hypoxic training; exposure to hypercapnic hypoxia combined with Mexidol; and monotherapy with Mexidol.Materials and methods. 60 male Wistar rats were used in the experiment. During 30 consecutive days, the animals underwent training cycles in the form of daily exposures to hypercapnic hypoxia (HCH) of submaximal intensity (O2 — 9%; CO2 — 7%); monotherapy with Mexidol; and cycles of hypercapnic hypoxic training of submaximal intensity combined with administration of Mexidol. Results: In the case of isolated repeated exposure to HCH of submaximal intensity, the response of the hemostatic system revealed itself through hypercoagulation at the final stage of coagulation and increased plasma fibrinogen concentrations. The anticoagulant activity of blood plasma was characterized by an increased level of AT III and APR (antithrombin plasma reserve). Activation of the fibrinolytic system was also observed. The 30-fold course administration of Mexidol was accompanied by a reduction in the platelet count and hypoaggregation. The multiple combined effects of HCH and Mexidol led to increase of anticoagulant and fibrinolytic activity in blood plasma. Conclusion. After repeated exposure to a moderate-intensity stimulus (HCH of submaximal intensity), an increase of certain hemostatic parameters (antithrombin III, fibrinogen) was observed, as well as an increase in ARP and plasma fibrinolytic activity, which can be considered as a manifestation of long-term adaptation of the hemostatic system to this type of stressor. The experiments revealed that repeated administration of Mexidol at a dose of 50 mg/kg also contributed to long-term adaptation of the hemostatic system and could be used as pharmacological preconditioning. The effects of multiple exposure to hypercapnic hypoxia combined with the antioxidant effects of the antihypoxic drug resulted in more evident adaptive changes in experimental rats: significant platelet hypoaggregation; normalization of the coagulation parameters of the hemostatic system to the level measured in control animals; disappearance of thrombotic readiness markers in the bloodstream, a significant increase in the anticoagulant and fibrinolytic activity of blood plasma, which contributes to a stable long-term adaptation of the hemostatic system.
Keywords: hypercapnic hypoxia, Mexidol, hemostatic system
Received 11.09.2019
For citation: Moskalenko S.V., Shakhmatov I.I., Kovalev I.V., Bondarchuk Yu.A., Alekseeva O.V., Ulitina O.M. Response of the Hemostatic System to to Repeated Cycles of Hypercapnic Hypoxic Training; Exposure to Hypercapnic Hypoxia Combined with Mexidol; and Isolated Treatment with Mexidol. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2019, Vol. 16, no. 3, pp. 330-341. DOI: 10.22138/2500-0918-2019-16-3-330-341 (In Russ)
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Authors
Moskalenko Svetlana Valerievna
Altai State Medical University
Assistant Professor of the Department of Normal Physiology, Junior Research Assistant
Russian Federation, Barnaul, 40, Prospekt Lenina 656038
State Scientific-Research Institute of Physiology and Basic Medicine
Russian Federation Novosibirsk, 4, Timakova Street, 630117
sunrisemsv@gmail.com
Shakhmatov Igor Ilyich
Altai State Medical University
Doctor of Medicine, Professor, Chief Researcher, Head of the Department of Normal Physiology
Russian Federation, Barnaul, 40, Prospekt Lenina 656038
State Scientific-Research Institute of Physiology and Basic Medicine
Russian Federation Novosibirsk, 4, Timakova Street, 630117
iish59@yandex.ru
Kovalev Igor Viktorovich
Siberian State Medical University
Doctor of Medicine, Рrofessor of the Department of Biophysics and Functional Diagnostics
Russian Federation 2 Moskovsky Trakt Tomsk 634055
kovalew@mail.ru
Bondarchuk Yulia Alekseevna
Altai State Medical University
Candidate of Medical Science, Associate Professor of the Department of Normal Physiology, Senior Researcher
Russian Federation, Barnaul, 40, Prospekt Lenina 656038
State Scientific-Research Institute of Physiology and Basic Medicine
Russian Federation Novosibirsk, 4, Timakova Street, 630117
bondarchuk2606@yandex.ru
Alekseeva Olga Vasilievna
Altai State Medical University
Candidate of Medical Science, Associate Professor of the Department of Normal Physiology, Senior Researcher
Russian Federation, Barnaul, 40, Prospekt Lenina 656038
State Scientific-Research Institute of Physiology and Basic Medicine
Russian Federation Novosibirsk, 4, Timakova Street, 630117
alekseeva0506@mail.ru
Ulitina Oksana Mikhailovna
Altai State Medical University
Candidate of Biological Sciences, Associate Professor of the Department of Normal Physiology, Junior Researcher
State Scientific-Research Institute of Physiology and Basic Medicine
Russian Federation Novosibirsk, 4, Timakova Street, 630117
oulitina@mail.ru