Dynamics of bone resorption markers upon implantation nanostructured сoated needles in osteoporotic rebuild bone
DOI: 10.22138/2500-0918-2016-15-3-33-38
A.Yu. Burmatova, E.B. Trifonova, V.N. Meshchaninov
Chaklin Ural Institute of Traumatology and Orthopedics, Yekaterinburg, Russian Federation;
Ural State Medical University, Yekaterinburg, Russian Federation
Abstract.
The quality improvement of the osteoreparation on the background of immobilization osteoporosis is actual task. The aim of the study was to examine the of osteogenesis features in using implants with nanostructured coatings and in terms of immobilization osteoporosis in experiment. On male Wistar rats modulated immobilization osteoporosis by resection of the right hind tibia in its proximal part. After the appearance of immobilization osteoporosis nanostructured composite (CN0,25)-coated and nanostructured diamond-like (a-C)-coated needles were implanted in osteoporotic rebuilt bone. The dynamics of bone resorption markers RatTRAP, RatLaps, PINP, pro-MMP-1, TIMP-1 with the calculation of the integral indexes were determined in serum before implantation, on terms of 30, 60 and 90 days after it. Comparative analysis of the studied parameters revealed a more pronounced changes in the bone resorption markers during implantation needles with CN0,25-coated needles. This group showed higher levels of osteoclast activity metabolic markers RatTRAP and pro-MMP-1, integral index RatLaps / PINP, whereas lower levels of TIMP1 / pro-MMP1 index. Obtained results probably illustrates differences in bone matrix degradation mechanisms that may be caused by a different surface topography of nanofilms.
Keywords:
RatTRAP, RatLaps/PINP, pro-ММP-1, TIMP-1, immobilization osteoporosis, nanostructured coatings
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Authors
Trifonova Elena B.
Chaklin Ural Institute of Traumatology and Orthopedics
620014, Russian Federation, Yekaterinburg,
per. Bankovskij, 7
trifonlab@mail.ru
Meshchaninov Victor N.
Institute for Medical Cell Technologies
Ural State Medical University
22a, Karla Marksa str., Yekaterinburg, Russian Federation, 620026
mv-02@yandex.ru
Burmatova Aleksandra Y.
Chaklin Ural Institute of Traumatology and Orthopedics
Ural State Medical University
620014, Russian Federation, Yekaterinburg,
per. Bankovskij, 7
alex.burmatova@mail.ru
Received — 29.07.2016
For citation:Burmatova A.Yu., Trifonova E.B., Meshchaninov V.N. Dynamics of bone resorption markers upon implantation nanostructured сoated needles in osteoporotic rebuild bone. Vestn. Ural. Med. Akad. Nauki. – Journal of Ural Medical Academic Science. 2016, no. 3, pp. 33–38. DOI: 10.22138/2500-0918-2016-15-3-33-38 [In Russ.]
