COMPARATIVE EXPERIMENTAL STUDY OF REGENERATIVE, ANTI-INFLAMMATORY AND ANTIMICROBIAL PROPERTIES OF TETRADERM PREPARATIONS AND TRIDERM
DOI: 10.22138/2500-0918-2023-20-1-63-74
K.I.Stosman¹, K.V. Sivak¹, T.N. Savateeva-Lyubimova¹, A.G. Aleksandrov¹,
N.V. Marchenko², S.A. Kopatko², I.V. Sychkova², D.R. Kargopoltseva²
¹Federal State Budgetary Institution «Smorodintsev Research Institute of Influenza” of the Ministry of Health of the Russian Federation, St. Petersburg, Russian Federation;
²JSC “WERTEKS”, St. Petersburg, Russian Federation
Abstract. The aim of study was to conduct the comparative regenerative, anti-inflammatory and antimicrobial properties of tetraderm and triderm in experiment. Materials and methods. Experiment was performed on 85 white male rats. Investigational medicines were applied 2 times a day at a dose of 0.01 g/cm2. The pharmaceuticals were applied in prophylactic scheme (2 and 1 hours before the induction of inflammation) and therapeutic scheme (1 hour after the induction of inflammation) for 7 days for the treatment of carrageenan-induced inflammation. Tetraderm and triderm were applied 24 hours after the thermal burn to the affected area of the skin during 21 days. Treatment of linear wounds was performed for 7 days 24 hours after surgery. The antibacterial and antifungal effects of drugs were evaluated in vitro. Results. Tetraderm reduced the limb edema by more than 50% in a prophylactic scheme compared with triderm. The anti-inflammatory effect of pharmaceuticals was comparable. Tetraderm shown pronounced regenerative ability and reduced the burn area by 1,5 times compared with triderm. The stimulating healing effect of tetraderm on the skin wound of the linear skin wound model was similar to that of triderm. The antimicrobial activity of the tetraderm and triderm was comparable. Tetraderm showed a fungicidal effect against yeast and mold fungi. Triderm had only a fungistatic effect against yeast С. albicans and suppressed of the growth of A. Niger. Conclusions. The regenerative and anti-inflammatory effect of tetraderm with antibacterial activity shows auspicious conditions for healing wounds and burns. The regenerative, antibacterial and fungicidal effects of tetraderm are slightly superior to the triderm effect.
Keywords: exudative inflammation, thermal burn, linear wound, antimicrobial and fungicidal effect, rats
There is no conflict of interest.
Contact details of the corresponding author:
Kira I. Stosman
labtox6@rambler.ru
Received 15.02.2023г
For citation:
Stosman K.I., Sivak K.V., Savateeva-Lyubimova T.N., Aleksandrov A.G., Marchenko N.V., Kopatko S.A., Sychkova I.V., Kargopoltseva D.R. Comparative experimental study of regenerative, anti-inflammatory and antimicrobial properties of Tetraderm preparations and Triderm. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2023, Vol. 20, no. 1-2, pp. 63–74. DOI: 10.22138/2500-0918-2023-20-1-63-74 (In Russ)
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Authors
Kira I. Stosman
Federal State Budgetary Institution «Smorodintsev Research Institute of Influenza” of
the Ministry of Health of the Russian Federation
Candidate of biological science
Senior Researcher, Drug Safety Laboratory
labtox6@rambler.ru
St. Petersburg, Russian Federation
Konstantin V. Sivak
Federal State Budgetary Institution «Smorodintsev Research Institute of Influenza” of
the Ministry of Health of the Russian Federation
Candidate of biological science
Head of Preclinical Research Department
kvsivak@gmail.com
St. Petersburg, Russian Federation
Tatiana N. Savateeva-Lyubimova
Federal State Budgetary Institution «Smorodintsev Research Institute of Influenza” of
the Ministry of Health of the Russian Federation
Doctor of medical science, Professor
Lead Researcher, Drug Safety Laboratory
drugs_safety@mail.ru
St. Petersburg, Russian Federation
Andrey G. Aleksandrov
Federal State Budgetary Institution «Smorodintsev Research Institute of Influenza” of
the Ministry of Health of the Russian Federation
Candidate of biological science
Researcher, Drug Safety Laboratory
forphchemistry@gmail.com
St. Petersburg, Russian Federation
Natalia V. Marchenko
Vertex JSC
Candidate of biological science, Associate Professor
Director of Science and Clinical Research
NMarchenko@vertex.spb.ru
St. Petersburg, Russian Federation
Svetlana A. Kopatko
Vertex JSC
Deputy director general for the product portfolio
SKopatko@vertex.spb.ru
St. Petersburg, Russian Federation
Irina V. Sychkova
Vertex JSC
Senior pharmacologist
ISychkova@vertex.spb.ru
St. Petersburg, Russian Federation
Dinara R. Kargopoltseva
Vertex JSC
Senior pharmacologist
DKargopoltseva@vertex.spb.ru
St. Petersburg, Russian Federation