Phenotypic and functional characteristics of regulatory T-cells (treg) in patients with colorectal cancer

DOI: 10.22138/2500-0918-2019-16-2-165-169

A.V. Novitskaia, A.V. Churov, A.A. Romanov

Institute of Biology of Karelian Research Centre Russian Academy of Sciences (IB KarRC RAS), Petrozavodsk, Russian Federation;
State Budgetary Institution of Healthcare «Republican Oncology Center», Petrozavodsk, Russian Federation

Abstract. The aim of the work was to study the phenotypic and functional characteristics of CD4+ T cells, primarily regulatory T lymphocytes (Treg) and their role in the formation of immune suppression mechanisms in CRC, as well as in acute pancreatitis (AP). Materials and methods. Analysis of the expression of tumor infiltrating lymphocytes (TIL) and blood lymphocytes was performed by flow cytometry. Determination of the messenger RNA (mRNA) of the CD39 gene in the leukocyte fraction of peripheral blood was carried out in real time by the method of polymerase chain reaction (PCR). Results. An accumulation of peripheral CD39+CD4+ T cells in the later stages of CRC was observed. Among TILs, the presence of CD4+CD39+ T cells was higher than in blood samples obtained from the same patients. A high level of CD39 expression was determined in Treg cells obtained both in the periphery and among TILs of patients with CRC. Significant associations between the content of CD4+CD39+ T-cells and the indicators of cellular immunity in patients with colorectal cancer were determined. Conclusions. Thus, the data obtained demonstrate the activation of CD4+CD39+ T cells in CRC patients. The funding: financial support for research was carried out from a grant from the Russian Science Foundation (project No. 17-75-10182).

Keywords: colorectal cancer; CD39; Treg cells; FOXP3; lymphocytes; acute pancreatitis

Received 15.04.2019.

For citation:
Novitskaia A.V., Churov A.V., Romanov A.A. Phenotypic and functional characteristics of regulatory T-cells (treg) in patients with colorectal cancer. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2019, Vol. 16, no. 2, pp. 165–169. DOI: 10.22138/2500-0918-2019-16-2-165-169 (In Russ)

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Authors
Anastasija V. Novitskaia
IB KarRC RAS
Senior biologist, Immuunology group
Pushkinskaya str. 11 Petrozavodsk Republic of Karelia Russian Federation 185014

Aleksei V. Churov
IB KarRC RAS
Cand. Sci. (Biology), Senior researcher, Immuunology group
Pushkinskaya str. 11 Petrozavodsk Republic of Karelia Russian Federation 185014
achurou@yandex.ru

Aleksander A. Romanov
Republic Oncology Dispensary
Oncology surgeon
Lososinskoye Highway 5 Petrozavodsk, Republic of Karelia, Russian Federation, 185002

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)