Hemoglobin response of the body to immobilization stress
S. A. Brilliant 1,2, B. G. Yushkov 1,2
1Institute of Medical Cell Technologies, Yekaterinburg, Russian Federation;
2Institute of Immunology and Physiology UB RAS, Yekaterinburg, Russian Federation
Abstract. It is known that changes in the ratio of peripheral blood hemoglobin fractions under the influence of an extreme factor on the body (acute massive blood loss, hepatectomy and others) are based on the restructuring of erythropoiesis. However, the question of the properties of individual fractions, of changes in the hemoglobin profile of the bone marrow remains unclear. The mechanisms are not clear, which regulate the ratio of hemoglobin fractions of the bone marrow and peripheral blood under stress. Objective: study the hemoglobin response of the body to immobilization stress. Materials and methods. The studies were carried out on male Wistar rats weighing 250-300 g. The model of neuromuscular tension proposed by H. Selye (immobilization of animals on the operating table on the back) was used as a stress factor once for 6 hours. All animals were divided into three groups: the first group consisted of intact rats, the second — rats that were withdrawn from the experiment 6 h after exposure, and the third group — animals that were withdrawn from the experiment 2 days after immobilization stress. In all experimental groups, the following parameters were assessed: complete blood count, the content of reticulocytes and erythrocytes with fetal forms of hemoglobin. The ratios of the protein fractions of hemoglobin of the bone marrow and peripheral blood were investigated by polyacrylamide gel electrophoresis according to G. Maurer. Results of research. We noted an increase in the total number of erythrocytes in the peripheral blood after immobilization stress in comparison with the group of intact animals. The number of reticulocytes and cells carrying fetal forms of hemoglobin increased for both periods after exposure. Under conditions of immobilization stress, a change in the ratio of individual protein fractions of hemoglobin in the bone marrow and peripheral blood was observed. At 6 h after immobilization stress, an increase in 3, 6 protein fractions and a decrease in 1, 2 hemoglobin isoforms were noted in the bone marrow. In peripheral blood, similar changes were noted. The total hemoglobin content in the bone marrow significantly decreased 2 days after the extreme exposure. A decrease in protein fractions 1, 2, 3, and 5 occurred, which is possibly due to the release of young (large) cells from the bone marrow into the bloodstream to compensate and restore the general state of the body after stress. Conclusions. Thus, immobilization stress leads to the release of young (large) erythroid cells from the bone marrow and the activation of erythropoiesis, as evidenced by reticulocytosis. The activation of erythropoiesis is characterized by the formation and entry into the peripheral blood of erythrocytes containing oxygen-dependent hemoglobin isoforms, which leads to a change in the ratio between the individual protein fractions of hemoglobin in the bone marrow and peripheral blood.
Keywords: hemoglobin isoforms, fetal hemoglobin, bone marrow, immobilization stress
There is no conflict of interest.
Contact details of the corresponding author:
Svetlana A. Brilliant
svetlana.brilliant@bk.ru
Received 26.11.2020
For citation:
Brilliant S.A., Yushkov B.G. Hemoglobin response of the body to immobilization stress. http://vestnikural.ru/article/1148 [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2020, Vol. 17, no. 4, pp. 266–271. DOI: 10.22138/2500-0918-2020-17-4-266-271 (In Russ)
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Authors
Svetlana A. Brilliant
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences (IIF UB RAS)
Junior researcher
106, Pervomayskay street, Yekaterinburg, Russian Federation, 620049
GAUZ SO Institute of Medical Cellular Technologies
Researcher science
22a, K. Marks str., Yekaterinburg, Russian Federation, 620026
svetlana.brilliant@bk.ru
Boris G. Yshkov
Institute of immunology and physiology of URO Russian Academy of Sciences (IIF UB RAS)
Head of the laboratory of immunophysiology and immunopharmacology, Dr. Sci. (Med.), Professor, Honored worker of science of the Russian Federation
106, Pervomayskay street, Yekaterinburg, Russian Federation, 620049
GAUZ SO Institute of Medical Cellular Technologies
Leading Researcher
22a, K. Marks str., Yekaterinburg, Russian Federation, 620026
b.yushkov@iip.uran.ru