GENETIC RISK FACTORS FOR ALLERGIC RESPIRATORY DISEASES
DOI: 10.22138/2500-0918-2025-22-4-379-391 (In Russ)
M.S. Dolgopolov, V.A. Sabynych, A.E. Yamshchikova, P.O. Reznichenko, E.V. Prosekova
Federal State Budgetary Educational Institution of Higher Education
«Pacific State Medical University» of the Ministry of Health of the Russian Federation, Vladivostok, Russian Federation
Abstract. Studies of genetic factors as early causes of allergic diseases are promising, but are complicated by the heterogeneity of the pathological process. Among children with allergic lesions, in comparison with a group of healthy peers, the chances of realizing the allergic phenotype of bronchial asthma with allergic rhinitis were calculated. The structural features and occurrence of single nucleotide polymorphisms of cytokine genes involved in the regulation of T2 and non-T2 inflammation were analyzed. The aim of the study was to find an associative relationship between polymorphic variants of cytokine genes and the risk of developing allergic respiratory diseases and cytokine imbalance in the pediatric population. Materials and methods. DNA from peripheral venous blood was used for the molecular genetic study of cytokine gene polymorphisms. The enzyme immunoassay was used to account for the content of total and specific immunoglobulin E and cytokines in blood serum. Mutational changes in key genetic loci of the following interleukins were selected: IL2 rs2069762 (T-330G); IL4 rs2243250 (C-589T); IL6 rs1800795 (C-174G); IL10 rs1800896 (G-1082A), IL10 rs1800871 (C-819T), IL10 rs1800872 (C-592A); IL17A rs2275913 (G-197A) and IL17F rs763780 (T-7488G) and cytokines, respectively: IFNy rs2430561 (T-874A) and TNFa rs1800629 (G-308A), respectively. The results of the study recorded a varying degree of association of the genotype with the content of cytokines, as well as IgE in the blood serum. A comparative analysis of the frequency and spectrum of cytokine polymorphisms of various Th profiles revealed additional genetic risk factors for allergic respiratory damage in children with variations depending on the combination of allergic diseases.
Keywords: children's population, cytokine genotypes, risk factors, single nucleotide polymorphisms, allergic diseases, asthma, rhinitis
There is no conflict of interest.
Contact details of the corresponding author:
Maxim S. Dolgopolov
gades.med@mail.ru
Recieved 30.04.2025
For citation: Dolgopolov M.S., Sabynych V.A., Yamshchikova A.E., Reznichenko P.O., Prosekova E.V. Genetic risk factors for allergic respiratory diseases [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2025, Vol. 22, no. 4, pp. 379–391. DOI: 10.22138/2500-0918-2025-22-4-379-391 (In Russ)
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Auhtors
Maxim S. Dolgopolov
Candidate of Medical Sciences, Associate Professor of the Department of Clinical Laboratory Diagnostics, General and Clinical Immunology
gades.med@mail.ru
Vitaly A. Sabynych
Candidate of Medical Sciences, Associate Professor, Associate Professor of the Department of Clinical Laboratory Diagnostics, General and Clinical Immunology
Anastasia E. Yamshchikova
Senior Laboratory Assistant, Resident of the Department of Clinical Laboratory Diagnostics, General and Clinical Immunology
Polina O. Reznichenko
Resident of the Department of Clinical Laboratory Diagnostics, General and Clinical Immunology
Elena V. Prosekova
Doctor of Medical Sciences, Professor, Head of the Department of Clinical Laboratory Diagnostics, General and Clinical Immunology
Pacific State Medical University of the Ministry of Health of the Russian Federation (PSMU of the Ministry of Health of the Russian Federation)
Vladivostok, Russian Federation