Hepatoprotective Activity of Miliacin in Methotrexate-Induced Liver Damage
Kalinina O.V., Frolov B.A., Shtil A.A.
SBEI of HPE Orenburg state medical academy, Orenburg, Russian Federation; FSBI N.N. Blokhin Russian Cancer Research Center RAMS, Moscow, Russian Federation
Abstract. Hepatoprotective influence of panic oil triterpenoids — miliacin in toxic liver damage caused by methotrexate (MTX) was studied during the experiment. Miliacin in a single dose of 2 mg/kg t.i.d. (over the period of 3 days) restricted structural damage of the organ, reduced hyperenzymemia and disorder of pigmentary exchange in mice (CBxC57Bl6)F1. Miliacin prevented clearance function depression of Kupffer’s cells in Wistar rats and increased anticancer effect of MTX in terms of tumor growth inhibition and survival rate of BDF1 mice with Lewis lung carcinoma. Protective effect of miliacin was mediated by restriction of oxidative stress and prooxidative gene — cyp 2e1 expression normalization in hepatocytes and gene antioxidative proteins — glured.
Keywords: cytostatic agents, hepatotoxicity, triterpenoids, hepatoprotectors
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Authors
Kalinina OlgaV.
SBEI of HPE Orenburg state medical academy, Orenburg, Russia
PhD
Assistant of the Department of Pathophysiology
Russian Federation, 460000, Orenburg, Parkoviy prospect, 7
Frolov Boris A., e-mail: raiamak@mail.ru
SBEI of HPE Orenburg state medical academy, Orenburg, Russia
MD, professor
The Head of the Department of Pathophysiology
Shtil Alexander A., e-mail: shtilaa@yahoo.com
FSBI N.N. Blokhin Russian Cancer Research Center RAMS, Moscow, Russia
MD
The Head of the Laboratory of cancer cell death mechanism
Russian Federation, 115478, Moskow, Kashirskoye shosse, 24