STUDY OF IMMUNE HOMEOSTASIS IN RESIDENTS OF THE NORTH-WESTERN AND MOUNTAIN-SOUTHERN REGIONS

DOI: 10.22138/2500-0918-2023-20-1-40-52

L.S. Shchegoleva¹, M.S. Kabbani¹,², T.B. Sergeeva¹,
E.Yu. Shashkova¹, O.E. Philippova¹

¹ N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch
of the Russian Academy of Sciences, Arkhangelsk, Russian Federation;
² Northern (Arctic) Federal University named after M.V. Lomonosov,
Arkhangelsk, Russian Federation

Abstract. Phenotypic features of human immune homeostasis in different climatic and geographical regions are determined by the characteristic formation of an adaptive immune response in changing environmental conditions. The purpose of the work — identification of variants of adaptive immune response using multidimensional statistical analysis in people of working age living in the extreme climate of the North-Western and Mountain-Southern regions. Materials and methods. The results of the survey of 164 people aged 20-60 years, residents of the North-Western region were analyzed: men — 38,42% (63 people), women – 61,59% (101 people). The content of monocytes, eosinophils, neutrophils, and lymphocyte phenotypes CD3+, CD4+, CD5+, CD8+, CD10+, CD16+, CD22+, CD71+, CD95+, HLA-DR+ were determined. The method of factor analysis was used to identify latent factors in the structure of immune status parameters. The classification of the subjects into groups with similar changes in a number of the considered indicators was performed using cluster analysis by the k-means method. Results: The clustering results showed that the state of the adaptive immune response of the subjects is characterized by 3 variants (in the North-Western) and 2 variants (Mountain-Southern) regions. Results. In the Northerners, the predominance of cell-mediated reactions associated with high functional activity of CD71+, HLA-DR+ cells was revealed, with factor 1 accounting for 40,98% of the total variance of signs, factor 2 accounting for 22,14%, and factor 3 — 12,95%, with a total share of the accumulated variance of signs of 76,07%. In the inhabitants of the Mountain-Southern region, the share in the total variance of signs is significantly less and in the total share of accumul Output.The most significant immunological indicators in the development and formation of an adaptive immune response, regardless of the region of residence, are the levels of antigens of the main histocompatibility complex class II HLA-DR+ and activated T-lymphocytes with the transferrin receptor CD71+.

Keywords: Cluster analysis, factor analysis, adaptive immune response, north-west, mountain-southern region, functional activity of lymphocytesated variance is only 61,94% against the background of low activity of CD71+, HLA-DR+

There is no conflict of interest.
Contact details of the corresponding author:
Tatyana B. Sergeeva
Tanya — 86@mail.ru
Received 02.02.2023г
For citation:
Shchegoleva L.S., Kabbani M.S., Sergeeva T.B., Shashkova E.Yu., Philippova O.E. Study of immune homeostasis in residents of the North-Western and Mountain-Southern regions. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2023, Vol. 20, no. 1-2, pp. 40–52. DOI: 10.22138/2500-0918-2023-20-1-40-52 (In Russ)

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Authors
Lyubov S. Shchegoleva
N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences
Doctor of Biological Sciences, Professor
Chief Researcher, Head of the Department laboratory of Physiology of Immunocompetent cells
Shchegoleva60@mail.ru
Arkhangelsk, Russian Federation

Mohammad S. Kabbani
N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences
Researcher at the Laboratory of Immunocompetent Cell Physiology
Northern (Arctic) Federal University named after M.V. Lomonosov
sohibmsk@hotmail.ru
Arkhangelsk, Russian Federation

Tatyana B. Sergeeva
N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences
Candidate of biological sciences
Researcher of the Laboratory of Physiology of Immunocompetent Cells Physiology
tanya — 86@mail.ru
Arkhangelsk, Russian Federation

Elizaveta Yu. Shashkova
N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences
Candidate of biological sciences
Researcher at the Laboratory of Immunocompetent Cell Physiology
eli1255@yandex.ru
Arkhangelsk, Russian Federation

Oksana E. Philippova
N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences
Candidate of biological sciences
Researcher, Laboratory of Immunocompetent Cell Physiology
oxana_filippova_85@mail.ru
Arkhangelsk, Russian Federation