RESEARCH HETEROGENEITY OF HEMOGLOBIN PROFILE THE BONE MARROW AFTER THE INFLAMMATORY PROCESS
DOI: 10.22138/2500-0918-2020-17-1-18-25
S.A. Brilliant, B.G. Yushkov, N.V. Tyumentseva
nstitute of Medical Cellular Technologies, Yekaterinburg, Russian Federation;
Institute of Immunology and Physiology, Yekaterinburg, Russian Federation
Abstract. The hemoglobin profile of peripheral blood is connect with a change in the ratio between individual populations of red blood cells under extreme effects on the body. However, shifts in the ratio of protein fractions hemoglobin of bone marrow in the development of inflammatory reactions remain poorly understood. The aim of the work was to study the heterogeneity of hemoglobin profile the bone marrow after the initiation of the inflammatory process. Materials and research methods. The experiments were performed on 50 Wistar rats. Acute aseptic inflammation was caused by introducing 0.5 ml of turpentine under the skin of the back. Animals were divided into the following groups: 1 group — control. Groups 2–4 initiated acute aseptic inflammation for periods of 6 h, 2 and 4 days uniformly. The following indicators were evaluated: myelogram, complete blood count, reticulocyte count and erythrocyte count with fetal forms of hemoglobin, the ratio of protein fractions of hemoglobin of bone marrow and peripheral blood. Results. After the initiation of the inflammatory process for 6 h, an increase in the total number of erythroblasts, myelocytes, reticular cells and megakaryocytes is noted in the myelogram in rats. An increase in the number of erythroblasts with an increase in the number of reticular cells indicates the activation of erythropoiesis at 6 h after the administration of turpentine. On the 2nd day after the development of the inflammatory reaction, the number of erythroblasts and myeloblasts is significantly reduced, in comparison with the intact group. On the 4th day there is an increase in the total number of myelocaryocytes due to an increase in cells of all types of neutrophilic and lymphoid series. There is a decrease in erythroblasts and megakaryocytes compared with the control. After turpentine is introduced for 6 h into the hemoglobin profile of the bone marrow, it is characterized by a decrease in the proportion of hemoglobin’s 1 and 2 of protein fractions due to an increase in the content of hemoglobin’s 3 and 6 isoforms. On the 2nd day after acute aseptic inflammation, the level of 5 and 6 protein fractions of hemoglobin increases due to a decrease in hemoglobin of 1, 2 and 3 protein fractions. On the 4th day after the initiation of the inflammatory reaction, a drop of 2, 3 isoforms is noted due to the growth of 4 and 5 protein fractions. Conclusion. The hemoglobin profile of the bone marrow characterized by an increase in fractions with acid-resistant properties. Neutrophils, coming from the bone marrow into the peripheral blood, migrate to the focus of inflammation and participate in the process of antimicrobial protection of the body. An increase in the number of reticular cells in the bone marrow during an inflammatory reaction indicates an increase in erythropoiesis.
Keywords: hemoglobin, bone marrow, inflammation
Received 10.12.2020
For citation: Brilliant S.A., Yushkov B.G., Tyumentseva N.V. Research heterogeneity of hemoglobin profile the bone marrow after the inflammatory process. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2020, Vol. 17, no. 1, pp. 18–25. DOI: 10.22138/2500-0918-2020-17-1-18-25 (In Russ)
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Authors
Svetlana A. Brilliant
Institute of Immunology and Physiology
Junior researcher
106 Pervomayskay str. Yekaterinburg Russian Federation 620049
Institute of Medical Cellular Technologies
Researcher science
22a Karl Marks str. Yekaterinburg Russian Federation 620026
svetlana.brilliant@bk.ru
Boris G. Yshkov
Institute of Immunology and Physiology
Dr. Sci. (Med.), Professor, Honored worker of science of the Russian Federation, Head of the laboratory of immunophysiology and immunopharmacology, Chief researcher
106 Pervomayskay str. Yekaterinburg Russian Federation 620049
Institute of Medical Cellular Technologies
Chief researcher
22a Karl Marks str. Yekaterinburg Russian Federation 620026
b.yushkov@iip.urun.ru
Natalya V. Tyumentseva
Institute of Immunology and Physiology
Senior researcher
106 Pervomayskay str. Yekaterinburg Russian Federation 620049
Institute of Medical Cellular Technologies
Cand. Sci. (Bio.), Leading researcher
22a Karl Marks str. Yekaterinburg Russian Federation 620026
tumen80@mail.ru