Changes in Morphometric Parameters of THE Spleen in Conditions of Exposure to Ionizing Izlucheniya after Stem Cell Transplant
DOI: 10.22138/2500-0918-2018-15-1-55-59
D.Yu. Grebnev, I.Yu. Maklakova, A.V. Osipenko
Ural State Medical University, Yekaterinburg, Russian Federation;
Institute of Medical Cell Technologies, Yekaterinburg, Russian Federation
Abstract. The aim of experimental research was to study the effect of combined transplantation of placental multipotent mesenchymal stromal cells and hematopoietic stem cells on morphometric parameters of the spleen in conditions of exposure to ionizing radiation. Materials and methods. Preparation of cell culture MMSC and GSK were made from the chorion of the placenta in laboratory animals. We studied the effects of ionizing radiation at a dose of 4.0 oz. The animals in the test subgroup was injected intravenously suspension MMSC and GSK respectively at a dose of 6 million cells/kg and 330 thousand cells/kg, the control sub-group was injected 0.9% NaCl — 0.2 ml intravenously. Intravenous administration was performed 1 hour after exposure once. Evaluation of regenerative processes in the red and white pulp of the spleen was carried out to determine the morphometric parameters. The results of the study. After exposure to ionizing radiation on the background of the introduction of stem cells marked by increase in size of lymphoid follicle due to the increase of the area of the B zone of the follicle. Also the increase in the density of cells in the red pulp of the spleen due to the increase in the content of erythroid cells and leukocytes, increasing the distance between the centers of lymphoid follicles. Conclusions. Combined transplantation of multipotent mesenchymal stromal and hematopoietic stem cells after exposure to ionizing radiation leads to the increase in the size of lymphoid follicles of the spleen, increasing the density of cells in red pulp.
Keywords: multipotent mesenchymal stromal cells, ionizing radiation, regeneration, spleen, morphometric parameters
Received 12.12.2017
For citation:
D.Yu. Grebnev, I.Yu. Maklakova, A.V. Osipenko. Changes in Morphometric Parameters of THE Spleen in Conditions of Exposure to Ionizing Izlucheniya after Stem Cell Transplant. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2018, Vol. 15, no. 1, pp. 55–59. DOI: 10.22138/2500-0918-2018-15-1-55-59 (In Russ)
REFERENCES
1. Sazonov S.V. T-lymphocytes — regulators of cell proliferation in tissue (scientific review). Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2007. No. 1. p. 91 (In Russ)
2. Jastrebov A.P., Grebnev D.Ju., Maklakova I.Ju. Stvolovye kletki, ih svojstva, istochniki poluchenija i rol' v regenerativnoj medicine. Ekaterinburg: UGMU 2016. 272 p. (In Russ)
3. Caplan A.I. Dennis J.E. Mesenchymal stem cells as trophic mediators. J. Cell Biochem. 2006; 98:1076-84.
4. Maklakova I. Yu., Yastrebov A. P., Grebnev D. Yu. Changing of the morphometric and cytological indexes of the spleen in the condition of acute blood loss on the background of stem cells insertion. Advances in Gerontology = Uspehi Gerontologii. 2015. V.28, no. 2. pp. 218–221. (In Russ)
5. Alvares-Silva M. Mouse placenta is a major hematopoietic organ. M. Alvares-Silva, P. Belo – Diabangouaya, J. Salaun, F. Dieterlen–Lievre. Developmental Cell. 2003. pp. 5437–5444.
6. Grebnev D.Y., Yastrebov A.P., Maklakova I.Y. The change of splenic morphometric parameters in aged laboratory animals exposed to ionizing radiation after undergoing stem cells transplantation. Kazan Medical Journal = Kazanskij medicinskij zhurnal. 2013. V. 94. №6: pp. 911-914. (In Russ)
7. Shahpazjan N.K. Sravnenie biologicheskih svojstv mul'tipotentnyh mezenhimal'nyh stromal'nyh kletok kostnogo mozga i placenty dlja klinicheskogo primenenija. N.K. Shahpazjan, T.A. Astrelina, M.V. Jakovleva, A.E. Gomzjakov, N.A. Novoselova, Ja.A. Kruglova, E.V. Bojakova, B.L. Lazebnik, O.V. Knjazev, E.V. Skorobogatova. Kletochnaja transplantologija i tkanevaja inzhenerija. 2012. V.7, No. 2. pp. 53-54. (in Russ)
8. Pilz, G.A. Human term placenta – derived mesenchymal stromal cells are less prone to osteogenic differentiation than bone marrow – derived mesenchymal stromal cells. G.A. Pilz, C. Ulrich, M. Ruh. Stem Cells and Development. 2011. Vol. 20, No. 4. pp. 635–646.
Authors
Dmitriy Yu. Grebnev
Ural State Medical University
Dr.Sci. (Med.), Associate Professor, Head of the Pathological physiology Department
Institute of Medical Cell Technologies
Senior Researcher
str. Repin 3, Yekaterinburg, 620028, Russian Federation
dr-grebnev77@mail.ru
Irina Yu. Maklakova
Ural State Medical University
Cand. Sci. (Med.), Associate Professor of the Department of Pathological physiology
Institute of Medical Cell Technologies
Senior Researcher
str. Repin 3, Yekaterinburg, 620028, Russian Federation
makliu@mail.ru
Arthur V. Osipenko
Ural State Medical University
Dr.Sci.(Med.), Professor, Department of Pathological physiology
str. Repin 3, Yekaterinburg, 620028, Russian Federation
dr-grebnev77@mail.ru