CHANGES IN T-CELL COMPARTMENTS OF THE WHITE PULP OF THE RAT SPLEEN IN THE POST-MECHANICAL INJURY PERIOD
DOI: 10.22138/2500-0918-2026-23-1-18-27
A. Kh. Lanicheva¹, V. V. Semchenko²
¹ Federal State Budgetary Educational Institution of Higher Education
“Bashkir State Medical University”, Ufa, Russian Federation;
² Federal State Budgetary Educational Institution of Higher Education
“Omsk State Agrarian University named after P. A. Stolypin”, Omsk, Russian Federation
Abstract. Any mechanical injury triggers a response in the body, including a local inflammatory response and a systemic response. The spleen's involvement in inflammatory responses is a complex and multifaceted process. Today, it is known that the spleen is a key immune organ, playing an active role in both systemic inflammation and the adaptive immune response, which manifests itself in the activation of T- and B-lymphocytes. Objective. Study of T-lymphocyte redistribution using CD-3 marker in different zones of the white pulp of the spleen after mechanical injury of the thigh in rats. Materials and methods. Modeling of mechanical injury of the hind limb in white rats, immunohistochemical method of verification of T-lymphocytes using labeled monoclonal antibodies to CD-3 with nuclei stained with Mayer's hematoxylin, statistical processing in the Statistica 7 program. Results. The number of T-lymphocytes increases sharply after 1 and 3 days in the mantle zone compared to the control group, which indicates a redistribution of the T-cell link in the early stages after injury, while the numerical density of T-lymphocytes in the PALM increases only after 7 days. The germinal center is suppressed after 7 and 14 days. Conclusions. The characteristic phasing of peak T-lymphocyte responses in different zones of the splenic white pulp indicates dynamic correlations that highlight the complex coordination between splenic compartments in the organization and resolution of the postreactive immune response.
Keywords: mechanical trauma, CD-3, T-lymphocytes, spleen
There is no conflict of interest.
Contact information of the author responsible for correspondence:
Received 26.11.2025
For citation: Lanicheva A.Kh., Semchenko V.V. Changes in T-cell compartments of the white pulp of the rat spleen in the post-mechanical injury period. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2026, Vol. 23, no. 1, pp. 18–27. DOI: 10.22138/2500-0918-2026-23-1-18-27 (In Russ)
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Authors
Albina Kh. Lanicheva
Federal State Budgetary Educational Institution of Higher Education “Bashkir State Medical University”
Associate Professor, Department of Histology, Ufa, Russian Federation
lanichevaa@mail.ru
orcid.org/0009-0004-0550-2760
Valery V. Semchenko
Federal State Budgetary Educational Institution of Higher Education “Omsk State Agar University named after P.A. Stolypin”, Omsk, Russian Federation
Professor, Department of Histology, Anatomy, Histology, Physiology and Pathological Anatomy
ivm_omgau_gistology@mail.ru
orcid.org/0009-0006-8933-9618