EXPERIMENTAL EVALUATION OF THE CYTOPROTECTIVE EFFECT OF PROBIOTIC METABOLITES OF BACILLUS SUBTILIS B-9909 STRAIN ON THE CULTURE OF ISOLATED HEPATOCYTES
N.A. Zabokritskiy
DOI: 10.22138/2500-0918-2022-19-3-203-209
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences, Yekaterinburg, Russian Federation
Abstract. The aim of the study was to study cytoprotective effect of BAS (metabolites produced by probiotic microorganisms of the genus Bacillus) of a sterile puffer culture of probiotic microorganisms of the Bacillus subtilis VCPM B-9909 on the culture of isolated hepatocytes when modeling their toxic damage. The preparation of a sterile puffer culture of Bacillus subtilis B-9909 was carried out by sterilizing filtration of the culture fluid of this strain. The work was carried out using a transferable L-41 cell culture line, which allows assessing the toxicity of various substrates on cell cultures and withstanding toxic effects in different concentrations. At the first stage of the research, the maximum non-toxic dose of sterile fugate and the minimum toxic dose of CCl₄ in relation to the cells of the L-41 culture were determined to solve the tasks set. At the next stage of research, cytoprotective effect of BAS, which are part fugate strain Bacillus subtilis B-9909, was studied and proved in relation to the cells of the L-41 line. cytoprotective and regenerative effects of BAS in the composition fugate strain Bacillus subtilis B-9909 were demonstrated on the model of toxic damage to the culture of isolated hepatocytes. In preclinical studies to assess the toxicity and safety of an experimental sample biohepatoprotector on experimental animals, it was found that the complex of biologically active substances (metabolites) administered intragastrically intraperitoneally is non-toxic and safe for laboratory animals and does not cause any pathological changes in their internal organs and tissues. The basis biohepatoprotector, which has a multifunctional mechanism of action that allows to effectively restore depressed liver functions, while simultaneously normalizing immunological parameters, is its active biocomponent — metabolites of probiotic spore-forming bacteria, which, when introduced into the body, produce a complex of biologically active metabolites (antibiotics, proteolytic, amylolytic, etc.enzymes, immunoglobulins, as well as interleukins, vitamins, proteins, amino acids, and others bioactive substances). The experimentally established cytoprotective effect of the complex of BAS, which are part fugate culture of Bacillus subtilis strain B-9909, will allow us to develop new promising medical immunobiological drugs that have a protective effect on human organs and tissues in the future.
Thus, as a result of the conducted studies, it was found that the BAS in the composition fugate of the Bacillus subtilis B-9909 strain not only have a pronounced cytoprotective effect, but also have a positive effect on the regenerative abilities of liver cells, which is a significant factor for the further use of this strain biocomponent immunotropic biohepatoprotector.
Keywords: metabolites, cytoprotector, probiotic microorganisms, cell culture, hepatocytes
There is no conflict of interest.
Contact details of the corresponding author:
Nikolai A. Zabokritskiy
pharmusma@rambler.ru
Received 10.06.2022
For citation:
Zabokritskiy N.A. Experimental evaluation of the cytoprotective effect of probiotic metabolites of Bacillus subtilis B-9909 strain on the culture of isolated hepatocytes. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2022, Vol. 19, no. 3, pp. 203–209. DOI: 10.22138/2500-0918-2022-19-3-203-209 (In Russ)
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Author
Nikolai A. Zabokritskiy
Institute of Immunology and Physiology, Ural Branch of RAS
МD, Associate Professor, Senior Researcher of the laboratory of immunophysiology and immunopharmacology
106 Pervomayskaya street Yekaterinburg Russian Federation 620049
pharmusma@rambler.ru
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