STUDY OF THE EFFECT OF COMBINED ALLOGENIC TRANSPLANTATION OF PLACENTAL MULTIPOTENT MESENHIMAL STROMAL AND HEMATOPOETIC STEM CELLS ON LIVER REGENERATION AFTER RESECTION
DOI: 10.22138/2500-0918-2020-17-1-80-88
V.V. Bazarnyi, I.Yu. Maklakova, D.Yu. Grebnev, I.V. Gavrilov, V.Ch. Vahrusheva
Ural State Medical University, Yekaterinburg, Russian Federation;
Institute of Medical Cell Technologies, Yekaterinburg, Russian Federation
Abstract. The aim of the study was to study changes in biochemical parameters of peripheral blood and liver morphometric parameters against the background of allogeneic combined transplantation of multipotent mesenchymal stromal cells (MMSC) and hematopoietic stem cells (HSC) after liver resection.
Materials and methods. The culture of MMSCs and HSCs was isolated from the placenta chorion of 5 laboratory animals of female mice 3-4 months old, weighing 22-23 g, gestational age 18 days. The mononuclear fraction of cells was obtained by sequential mechanical and enzymatic treatment of placental tissue. HSC was isolated by positive immunomagnetic separation by SCA-1 and CD 117 antigens. MMSC was cultured under conditions of a CO2 incubator at a temperature of 37 ° C with a carbon dioxide content of 5% and a humidity of 90%. For transplantation to laboratory animals, MMSCs were used at a dose of 4 million cells / kg and HSCs at a dose of 330 thousand cells / kg. The introduction of cells was carried out under physiological conditions (without liver resection) and 1 hour after subtotal liver resection. The biochemical indices of peripheral blood and morphometric indices of the liver were evaluated on days 1, 3, 7 after the administration of MMSC. Results. As a result of the study, it was found that the combined transplantation of MMSCs and HSCs after liver resection leads to a decrease in the level of cytolysis enzymes (AST, ALT, alkaline phosphatase), and an improvement in the protein synthesizing function of the liver (increase in total protein, albumin levels). Also, after the introduction of cells against the background of liver resection, an increase in the area of the hepatocyte nucleus, nuclear cytoplasmic index (NRC), and the number of binuclear cells was noted. An increase in the level of mitotic activity, a decrease in the severity of apoptosis, led to an increase in the number of hepatocytes. Conclusion. The restoration of the morphological and functional properties of the liver after its subtotal resection against the background of allogeneic combined transplantation of MMSCs and HSCs was found. This is expressed in improving the biochemical parameters of the peripheral blood of the liver, and morphometric parameters of the liver. In this case, activation of both cellular regeneration and intracellular was revealed.
Keywords: liver resection, multipotent mesenchymal stromal cells, hematopoietic stem cells, cell regeneration, intracellular regeneration
Received 10.12.2020
For citation: Bazarnyi V.V., Maklakova I.Yu., Grebnev D.Yu., Gavrilov I.V., Vahrusheva V.Ch. Study of the effect of combined allogenic transplantation of placental multipotent mesenhimal stromal and hematopoetic stem cells on liver regeneration after resection. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2020, Vol. 17, no. 1, pp. 80–88. DOI: 10.22138/2500-0918-2020-17-1-80-88 (In Russ)
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Authors
Vladimir V. Bazarnyi
Ural State Medical University
Dr. Sci. (Med.), Professor of the Department of clinical laboratory diagnostics and bacteriology,
3 Repin str. Yekaterinburg Russian Federation 620028
vlad-bazarny@yandex.ru
Irina Yu. Maklakova
Ural State Medical University
Cand. Sci. (Med.), Associate Professor of the Department of pathological physiology
3 Repin str. Yekaterinburg Russian Federation 620028
Institute of Medical Cell Technologies
Senior researcher
22a Karl Marx str. Yekaterinburg Russian Federation 620026
makliu@mail.ru
Dmitry Yu. Grebnev
Ural State Medical University
Dr. Sci. (Med.), Head of the Department of pathological physiology
3 Repin str. Yekaterinburg Russian Federation 620028
Institute of Medical Cell Technologies
Senior researcher
22a Karl Marx str. Yekaterinburg Russian Federation 620026
dr-grebnev77@mail.ru
Ilya V. Gavrilov
Ural State Medical University
Cand. Sci. (Bio.), Associate Professor
3 Repin str. Yekaterinburg Russian Federation 620028
Institute of Medical Cell Technologies
Senior researcher
22a Karl Marx str. Yekaterinburg Russian Federation 620026
given18@yandex.ru
Victoria Ch. Vahrusheva
Ural State Medical University
Postgraduate of the Department of Pathological Physiology
3 Repin str. Yekaterinburg Russian Federation 620028
yusupova1@inbox.ru