ABOUT CELLULAR REGULATION OF LIVER REGENERATION
DOI: 10.22138/2500-0918-2019-16-3-357-364
V.V. Bazarniy, I.Yu. Maklakova, D.Yu. Grebnev, V.Ch. Yusupova, E.M. Petrunina
Ural State Medical University. Ministry of Health of Russia, Ekaterinburg, Russian Federation;
Institute of Medical Cell Technologies, Ekaterinburg, Russian Federation;
Ural Research Institute of Phthisiopulmonology, Ekaterinburg, Russian Federation
Abstract. The aim of the work is to assess the possible use of cell technology activating liver regeneration from physiological conditions and after injury.Materials and methods. Various experimental models were used for transplantation of thymic cells or multipotent mesenchymal stromal cells (MSCs) to intact animals and animals after a partial hepatectomy. Liver regeneration was evaluated by morphological and biochemical parameters. MSCs were isolated from placenta chorion of 10 laboratory female mice aged 3-4 months, weighing 22-25 g, gestation period of 14 days. MSCs were grown in a standard cell culture incubator conditions — 37°C, 5% CO2, with carbon dioxide content of 5% and humidity of 90%. For transplantation to laboratory animals MSCs of passage 3 at a dose of 4 million cells/kg were used.Results. Transplantation of donor thymic cells after partial liver resection to intact recipients caused an increase in the number of binuclear hepatocytes and in DNA content in these hepatocytes. Iron deficiency led to a decrease in this ability of T-lymphocytes. Transplantation of MSCs to laboratory animals after hepatectomy led to an increase in the mitotic index, the number of binuclear hepatocytes, and the hepatocyte nuclear area. All these factors led to an increase of the nuclear-cytoplasmic ratio. After infusion of MSCs to rats with liver resection, the activity of the cytolitic enzymes that induce cell death decreased.Conclusion. The study revealed that lymphoid cells and MSCs are able to stimulate reparative processes in the liver. Increased cellular (mitotic activity) and intracellular (greater number of binuclear hepatocytes) regeneration were revealed, normalization of biochemical parameters after liver resection were discovered.
Keywords: liver regeneration, lymphocytes, multipotent mesenchymal stromal cells
Received 21.03.2019
For citation: Bazarniy V.V., Maklakova I.Yu., Grebnev D.Yu., Yusupova V.Ch., Petrunina E.M. About Cellular Regulation of Liver Regeneration. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2019, Vol. 16, no. 3, pp. 357-364. DOI: 10.22138/2500-0918-2019-16-3-357-364 (In Russ)
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Authors
Bazarniy Vladimir Viktorovich
Ural State Medical University of the Ministry of Health of Russia
MD, PhD, Professor of the Department of Clinical Laboratory Diagnostics and Bacteriology,
3 Repin St., Ekaterinburg, 620078, Russian Federation
vlad-bazarny@yandex.ru
Maklakova Irina Yuryevna
Ural State Medical University of the Ministry of Health of Russia
PhD in Medical Sciences, Senior Associate Professor of the Department of Pathological Physiology
3 Repin St., Ekaterinburg, 620078, Russian Federation
State Autonomous Institution of Health “Institute of Medical Cell Technologies”
Senior Researcher
21, Sobolev St., Ekaterinburg, 620026, Russian Federation
makliu@mail.ru
Grebnev Dmitry Yuryevich
Ural State Medical University of the Ministry of Health of Russia
PhD in Medical Sciences, Head of the Department of Pathological Physiology
3 Repin St., Ekaterinburg, 620078, Russian Federation
State Autonomous Institution of Health “Institute of Medical Cell Technologies”
Senior Researcher
21, Sobolev St., Ekaterinburg, 620026, Russian Federation
dr-grebnev77@mail.ru
Yusupova Victoria Chaukatovna
Ural State Medical University of the Russian Ministry of Health
Postgraduate of the Department of Pathological Physiology
3 Repin St., Ekaterinburg, 620078, Russian Federation
yusupova1@inbox.ru
Petrunina Ekaterina Mikhailovna
Ural Scientific Research Institute of Phthisiopulmonology – a branch of National Medical Research Center for Physiopulmonology and Infectious Diseases, Ministry of Health of Russia
Research Assistant
50, 22nd Parts’ezd St., Ekaterinburg, 620039, Russian Federation
ekaterina_b89@list.ru