OXYGEN TRANSPORT ABILITY OF ERYTHROCYTES IN CHRONIC PERIODONTITIS
DOI: 10.22138/2500-0918-2020-17-1-33-41
T.I. Vlasova, A.N. Sidorenko, E.V. Kondjurava, E.A. Tashina, V.V. Akimov, A.S. Fedos'kina, D.D. Kosynkina
National Research Ogarev Mordovia State University, Saransk, Russian Federation;
Federal State Budgetary Educational Institution of Higher Education «Kuban State Medical University», Krasnodar, Russian Federation
Abstract. Aim. To study the oxygen-transporting ability of red blood cells in chronic periodontitis. Materials and methods. Clinical studies of 34 patients with chronic generalized periodontitis: the first group (n=20) — periodontitis of moderate severity, the second group (n=16) — severe periodontitis. The content of lipid oxidation products, the activity of phospholipase A2, catalase, and the determination of the phospholipid composition of erythrocytes (densitometer GS-670 (BIO-RAD, USA); the conformation and properties of erythrocyte hemoglobin by Raman spectroscopy (in via Basis, Renishaw (UK)). Results. In chronic severe generalized periodontitis, the violation of the oxygen transport function of hemoglobin (a decrease in hemoglobin's ability to bind ligands by 18.57% (p<0.05) and a decrease in the hemoglobin's affinity to oxygen by 14.30% (p<0.05)) with conformational changes in pyrroles and hemoglobin in general was determined. These events correlated with changes in the phospholipid spectrum of the erythrocyte cell membrane and an increase in the content of lipid peroxidation products and erythrocyte phospholipase A2 activity. Under periodontitis of moderate severity, these changes are less pronounced. Conclusion. In chronic generalized periodontitis, there is dependence of pathological changes in the oxygen-binding ability of hemoglobin from the biochemical characteristics of erythrocyte membranes and the oxidative processes. Identified changes correlate with the severity of the disease.
Keywords: chronic generalized periodontitis, oxygen-binding ability of hemoglobin, red blood cells, oxidative processes, lipid composition
Received 10.12.2020
For citation:Vlasova T.I., Sidorenko A.N., Kondjurava E.V., Tashina E.A., Akimov V.V., Fedos'kina A.S., Kosynkina D.D. Ogen transport ability of erythrocytes in chronic periodontitis. Vestn. Ural. Med. Akad. Nauki.=Journal of Ural Medical Academic Science. 2020, Vol. 17, no. 1, pp. 33–41. DOI: 10.22138/2500-0918-2020-17-1-33-41 (In Russ)
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Authors
Tatyana I. Vlasova
National Research Ogarev Mordovia State University
Dr. Sci. (Med.), Аssociate Professor, Professor of the Department of Normal and Pathological Physiology
68 Bolshevitskaya str. Saransk Russian Federation 430005
vlasova.tatyanka@mail.ru
Alexander N. Sidorenko
Kuban State Medical University
Dr. Sci. (Med.), associate professor, associate Professor, Department of Orthopedic Dentistry
4 M. Sedina str. Krasnodar Russian Federation 350063
info@rosminzdrav.ru
Evgenia V. Kondyurova
National Research Ogarev Mordovia State University
Cand. Sci. (Med.), Associate Professor, Head of the Department of Dentistry
68 Bolshevitskaya str. Saransk Russian Federation 430005
Elena A. Tashina
National Research Ogarev Mordovia State University
Postgraduate student, Department of Dentistry
68 Bolshevitskaya str. Saransk Russian Federation 430005
Vladimir V. Akimov
National Research Ogarev Mordovia State University
Applicant for the Department of Dentistry
68 Bolshevitskaya str. Saransk Russian Federation 430005
Anna S. Fedoskina
National Research Ogarev Mordovia State University
5th year student of the Medical Institute
68 Bolshevitskaya str. Saransk Russian Federation 430005
Daria D. Kosykina
National Research Ogarev Mordovia State University
6th year student of the Medical Institute
68 Bolshevitskaya str. Saransk Russian Federation 430005