CLINICAL ASSESSMENT OF MORPHOLOGICAL AND FUNCTIONAL FEATURES OF NEUTROPHILS IN ISCHEMIC HEART DISEASE
DOI: 10.22138/2500-0918-2018-15-5-684-690
V.V. Bazarnyi, E.A. Partylova, Yu.V. Shilko, K.V. Kondrashov
Ural State Medical University, Yekaterinburg, Russian Federation;
Sverdlovsk regional clinical hospital N1, Yekaterinburg, Russian Federation
Abstract. The aim of the work is a comparative assessment of quantitative and functional parameters of blood neutrophils in patients with different forms of ischemic heart disease (IHD). Materials and methods. In patients with acute myocardial infarction and IHD disease, the number of neutrophils, the content of myeloperoxidase in them by flow cytometry, non-enzyme cationic proteins by cytochemical method, the level of markers of myocardial damage in immunochemical tests were determined. Results. An increase in the level of neutrophils, including band forms, with a decrease in the level of intracellular myeloperoxidase was established. These changes were more significant in acute myocardial infarction than in IHD. The positive correlation of the myeloperoxidase level and non-enzyme cationic proteins with troponin concentration is shown. Conclusion. The study of peripheral blood neutrophils by flow cytometry with the determination of intracellular myeloperoxidase can be considered as an additional tool for laboratory diagnosis of ischemic heart disease.
Keywords: neutrophils, myeloperoxidase, ischemic heart disease
Contact information of the author responsible for correspondence:
Bazarnyi Vladimir Viktorovich
vlad-bazarny@yandex.ru
Received 27.10.2018
For citation:
Bazarnyi V.V., Partylova E.A., Shilko Yu.V., Kondrashov K.V. Clinical assessment of morphological and functional features of neutrophils in ischemic heart disease. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2018, Vol. 15, no. 5, pp. 684–690. DOI: 10.22138/2500-0918-2018-15-5-684-690 (In Russ)
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Authors
Bazarnyi Vladimir V.
Ural State Medical University
Dr. Sci. (Med.), Professor of the Department of Clinical Laboratory Diagnostics and Bacteriology
Russian Federation, 620028, Yekaterinburg, Repina str., 3
vlad-bazarny@yandex.ru
Elena A. Partylove
Sverdlovsk Regional Clinical Hospital № 1
Cand. Sci. (Med.), doctor
Russian Federation, 620102, Yekaterinburg, Volgogradskaya str., 185
office@okb1.ru
Yulia V. Shilko
Sverdlovsk Regional Clinical Hospital № 1
Head of the Cardiology Department
Russian Federation, 620102, Yekaterinburg, Volgogradskaya str., 185
office@okb1.ru
Konstantin V. Kondrashov
Sverdlovsk Regional Clinical Hospital № 1
Cand. Sci. (Med.), Head of Cardiosurgery Department
Russian Federation, 620102, Yekaterinburg, Volgogradskaya str., 185
office@okb1.ru