ASSESSMENT OF THE RHEOLOGICAL STATE OF BLOOD BY CORRELATION OF MORPHOLOGICAL AND QUANTITATIVE PARAMETERS OF LEUKOCYTES DURING INTOXICATION OF RATS WITH COPPER-ZINC PYRITE ORE

DOI:10.22138/2500-0918-2024-21-1-22-32

A.R. Nikitina, K.R. Ziyakaeva, A.F. Kayumova, V.G. Shamratova

Bashkir State Medical University, Ufa, Russian Federation

Abstract.
Blood cells, along with performing specific functions, jointly control the rheological properties of blood. The role of erythrocytes and platelets in maintaining blood flow has been well studied, while the influence of leukocytes on the state of blood flow is given much less attention. At the same time, the size of leukocytes, the nucleus and the granularity of the cytoplasm contribute to the obstruction of blood flow in the capillaries, and an increase in their number can lead to a violation of rheology in the microcirculatory bed. Because of this, the establishment of links between the number of leukocytes and the volume in conditions of a significant increase in their number becomes of great importance, in particular when the body is intoxicated with heavy metal salts. The negative effects of exposure to natural salts of heavy metals on the body can also occur in people who come into contact with them at mining enterprises. The purpose of the study research was to study the relationship between the number and volume of leukocytes in the peripheral blood of rats exposed to copper-zinc pyrite ore. Materials and methods. The study was carried out on 60 non inbreding male rats aged three-months (average weight 210,5±10,5 g). In accordance with the timing of exposure to copper-zinc pyrite ore, the rats were divided into several groups. Blood parameters were analyzed on a hematological analyzer Vet Exigo 19 (Sweden). Results. From the first stage of the experiment (from the 20th day), an increase in the number was observed, and from the 30th day — and the volume of leukocytes. The increase in the content of granulocytes and monocytes continued in the subsequent periods of the experiment, while the volume of cells decreased slightly, approaching the initial level. Correlation analysis revealed positive correlations of quantitative and corpuscular parameters of granulocytes and monocytes on the 30th day and negative — granulocytes on the 45th day. The formation of negative reciprocal relations obviously reflects the action of regulatory mechanisms aimed at maintaining an optimal balance between the number and corpuscular volume of leukocytes.

Keywords:
rats, copper-zinc pyrite ore, heavy metals, leukocytes, cell volume, number of leukocytes

There is no conflict of interest.
Contact details of the corresponding author:
Albina R. Nikitina
nikitina.albinar@gmail.com
Received 22.09.2023
For citation:
Nikitina A.R., Ziyakaeva K.R., Kayumova A.F., Shamratova V.G. Assessment of the rheological state of blood by correlation of morphological and quantitative parameters of leukocytes during intoxication of rats with copper-zinc pyrite ore. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2024, Vol. 21, no. 1, pp. 22–32. DOI:10.22138/2500-0918-2024-21-1-22-32 (In Russ)

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Authors
Albina R. Nikitina
Assistant of the Department of Normal Physiology
nikitina.albinar@gmail.com
ORCID: https://orcid.org/0000-0001-8186-8204

Klara R. Ziyakayeva
Candidate of Biological Sciences
Associate Professor of the Department of Normal Physiology
klazia@yandex.ru
ORCID: https://orcid.org/0000-0002-3923-2736

Aliya F. Kayumova
Doctor of Medical Sciences, Professor
Head of the Department of Normal Physiology
norfiz@yandex.ru
ORCID: https://orcid.org/0000-0003-1983-1392

Valentina G. Shamratova
Doctor of Biological Sciences
Professor of the Department of Normal Physiology
distantshamratova@mail.ru
ORCID: https://orcid.org/0000-0002-7633-4264

Bashkir State Medical University
Ufa, Russian Federation