SOME METHODOLOGICAL TECHNIQUES FOR STUDYING PROLIFERATIVE PROCESSES IN TISSUE CELLS UNDER EXTREME IMPACTS ON THE ORGANISM
DOI: 10.22138/2500-0918-2022-19-5-474-484
S.V. Sazonov¹,²
¹Federal State Budgetary Educational Institution of Higher Education
“Ural State Medical University” of the Ministry of Health of the Russian Federation, Yekaterinburg, Russian Federation;
²GAUZ SO “Institute of Medical Cellular Technologies”, Yekaterinburg, Russian Federation
Abstract. When studying regeneration, it is often difficult to give an unambiguous answer to the question of whether the regenerative capacity in mammals decreases or increases under extreme effects on the body, especially when it comes to initially low values of the studied parameters. The assessment of regenerative processes implies the solution of several fundamental questions before conducting research. Ignoring them can lead to methodological miscalculations both in the planning of studies and directly when the results are obtained, and even more so in their subsequent analysis. Materials and methods. The assessment of regenerative processes at the cellular level was carried out using the morphometric method, DNA flow cytometry, autoradiography, calculation of the statmokinetic index, and immunohistochemical determination of Ki67 expression. An experimental model of liver regeneration after partial hepatectomy and resection of the small intestine was used. Results. It has been shown that the tissues entering the organ can regenerate in different ways and at different rates, which, apparently, determines the features and outcome of the regenerative process at the organ level. The identification of the main groups of tissues for the study of regeneration is simultaneously associated with the solution of the question not only of the level of the course of regenerative processes, but also of the necessary methodological techniques for their assessment. So, if the tissue planned for study has only the cellular level of regeneration, there is no need to study them at the intracellular level, and the study of regeneration in this case is limited to assessing the manifestations of mitotic activity in its cells. Methods for studying cell proliferation and requirements for their use are defined.
Keywords: regeneration, cell proliferation, research methods, models of reparative regeneration, experiment planning, extreme exposure
There is no conflict of interest.
Contact details of the corresponding author:
Sergey V. Sazonov
Prof-SSazonov@yandex.ru
Received 01.12.2022
For citation:
Sazonov S.V. Some methodological techniques for studying proliferative processes in tissue cells under extreme impacts on the organism. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2022, Vol. 19, no. 5, pp. 474–484. DOI: 10.22138/2500-0918-2022-19-474-484 (In Russ)
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Author
Sergey V. Sazonov
Federal State Budgetary Educational Institution of Higher Education «Ural State Medical University» of the Ministry of Health of the Russian Federation
Head of the Department of Histology, Doctor of Medical Sciences, Professor
3 Repina str. Yekaterinburg Russian Federation 620028, usma@usma.ru
Prof-SSazonov@yandex.ru