A METHOD FOR ISOLATION OF MYCOBACTERIUM TUBERCULOSIS IN SANITARY-BACTERIOLOGICAL EVALUATION OF CONTAMINATION OF AIR AND SURFACES OF TB INSTITUTIONS
Mordovskoy G. G., Ponikarovskaya I. V., Yakushkina A. Y., Putyrsky V.P., Yampolskiy V. A., Panov G. V.
State Health Establishment of the Sverdlovsk region “TB dispensary”,
Yekaterinburg, Russian Federation;
RASTER LLC, Yekaterinburg, Russian Federation
Abstract.
The present paper discusses the results of the survey of 12 TB Establishments (TBE) of the Sverdlovsk region conducted within the framework of the sanitary and bacteriological control of the mycobacteria tuberculosis (MBT) in the air of the enclosed spaces. The air sampling was carried out using the “Ultraproba-R” sampler based on a powerful water filtration vacuum cleaner “Rainbow D” [1, 2]. Surface sampling swab was done using a sampler based on a “Thomas Twin XT” washing vacuum cleaner [3]. Sampling was carried out before and after the aerosol treatment of the premises by “Desargent” solution with 10 % hydrogen peroxide concentration and 3ml/m3 dosage, using “Ultrasprayer R-60” fine-dispersed sprayer.
The paper provides the data of bacteriological and molecular genetic methods (MGM) of air samples and swab analysis, and gives the comparative characteristic of the methods when exercising sanitary and bacteriological control.
The sanitary bacteriological analysis revealed that the luminescent microscopy test-sensitivity excels that of cultural method. However, only cultural method can confirm the vitality of mycobacteria (MB) — MBT and mycobacteria nontuberculous (MBNT). The authors also found out that — in comparison with a diagnostic study — it is necessary to considerably lengthen the incubation period when sanitary and bacteriological studies of the inoculation in a solid medium (SM) due to a reduced metabolic rate of MB cells persistent in difficult environments, and to a slower growth of MB culture.
The paper demonstrates the effectiveness of the aerosol treatment. The luminescent microscopy shows the increase of damaged MB cells, and the cultural study shows the lack of detection of viable MB.
Key words:
sampler, “Ultrasprayer R-60”, “Desargent”, luminescent microscopy, bacteriological study, molecular genetic study, mycobacteria tuberculosis
REFERENCES
1. Mordovskoj G.G., Putyrskij V.P., Jampol'skij V.A., Mordovskaja Z.I., Ustrojstvo dlja otbora prob vozduha v pomeshhenii. Patent na poleznuju model' RF, № 117183 ot 27.01.2012 g.
2. Mordovskoj G.G., Ponikarovskaja I.V., Jakushkina A.Ju., Putyrskij V.P., Jampol'skij V.A. Ustrojstvo dlja zabora prob vozduha v pomeshhenii dlja vyjavlenija mikobakterij tuberkuljoza. Ural'skij medicinskij zhurnal, № 10, 105-108, 2012 g.
3. Mordovskoj G.G., Putyrskij V.P., Jampol'skij V.A., Mordovskaja Z.I., Ustrojstvo dlja otbora prob mikobakterij s poverhnostej: Patent na poleznuju model' RF: № 133612 ot 25.03.2013.
4. Golubev D.N., Mordovskoj G.G., Egorova O.S. Sovremennye predstavlenija o nozokomial'nom tuberkuljoze u sotrudnikov protivotuberkuljoznyh uchrezhdenij: http://fiziopulmo.ru/fp/i/full/1_2011_11_sovremennye_.pdf
5. Jampol'skij V.A. Provedenie ajerozol'noj dezinfekcii pomeshhenij LPO s pomoshh'ju ajerozol'nyh generatorov. Poliklinika. 2012; 6: 52-53.
6. Instrukcija № 20/12 po primeneniju dezinficirujushhego sredstva «Dezargent» proizvodstva OOO «RASTER». Ekaterinburg, 2012; http://www.dezreestr.ru
Authors
Mordovskoy Georgiy G.
State Health Establishment of the Sverdlovsk region “TB dispensary”, Yekaterinburg, Russian Federation
M.D, Head of Bacteriological Laboratory
Ponikarovskaya IrinaV.
State Health Establishment of the Sverdlovsk region “TB dispensary”, Yekaterinburg, Russian Federation
Doctor
Yakushkina Alyona U.
State Health Establishment of the Sverdlovsk region “TB dispensary”, Yekaterinburg, Russian Federation
Biologist
Putyrsky V.P.
RASTER LLC, Yekaterinburg, Russian Federation
PhD, Director General
Yampolskiy Valeriy A.
yampolskij@yandex.ru
RASTER LLC, Yekaterinburg, Russian Federation
PhD, Head of Manufacturing Department
Panov Grigoriy V.
State Health Establishment of the Sverdlovsk region “TB dispensary”, Yekaterinburg, Russian Federation
Doctor
Lenina St. 79-а, apt. 24, 620075 Yekaterinburg, Russian Federation