Modeling of chronic periodontitis on laboratory animals
DOI: 10.22138/2500-0918-2018-15-4-563-569
V.V. Bazarnyj, L.G. Polushina, E.N. Svetlakova, S.Ju. Medvedeva,A.Yu. Maksimova, Е.V. Mandra
Urals State Medical University, Yekaterinburg, Russian Federation;
Institute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences, Yekaterinburg, Russian Federation
Abstract. The purpose of the work was to create an adequate model of chronic periodontitis in laboratory animals on the basis of modern ideas about the etiology and pathogenesis of this disease. The article presents a method for modeling chronic periodontitis in laboratory rats. It consists in using a combination of several etiological and pathogenetic factors — mechanical damage to tissues, bacterial contamination of the wound surface, violation of microcirculation of tissues. Materials and methods of research: 40 mature Wistar male rats were used in the experiment to create a periodontitis model. Morphological study was performed to assess inflammatory and reparative processes in periodontal tissues. A set of laboratory tests was used to study systemic reactions of the body. The results of the study showed that an adequate model of experimental periodontitis has been developed, this is confirmed by the revealed morphological changes in the periodontal tissues after a complex effect on them. Conclusion: The use of combined effects on periodontal tissues of laboratory animals, including mechanical damage (rupture of the circular ligament of the tooth), bacterial contamination of the wound surface, disturbance of microcirculation of tissues, leads to the development of chronic generalized periodontitis. Its morphological picture is similar to that of a human. This proves that we have received an adequate model of periodontitis. The maximum morphological shifts were detected in animals from 21 days to 35 days after the operation. There was no pronounced systemic blood reaction in this case.
Keywords: chronic periodontitis, laboratory animals, morphological study, complex of laboratory tests
Received 14.05.2018
For citation:
Bazarnyj V.V., Polushina L.G., Svetlakova E.N., Medvedeva S.Ju., Maksimova A.Yu., Mandra E.V. Modeling of chronic periodontitis on laboratory animals. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2018, Vol. 15, no. 4, pp. 563–569. DOI: 10.22138/2500-0918-2018-15-4-563-569 (In Russ)
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Authors
Vladimir V. Bazarnyi
Urals State Medical University
Dr. Sci. (Med.), Professor, Chief scientific officer of the Central scientific research laboratory
Russian Federation, 620028, Yekaterinburg, ul. Repina, 3
vlad-bazarny@yandex.ru
Larisa G. Polushina
Urals State Medical University
Scientific employee of the Central Research Laboratory
Russian Federation, 620028, Yekaterinburg, ul. Repina, 3
polushina-larisa@bk.ru
Elena N. Svetlakova
Urals State Medical University
Cand. Sci. (Med.), Assistant professor of the Preclinical dentistry department and physiotherapy of dental diseases
Russian Federation, 620028, Yekaterinburg, ul. Repina, 3
svet_anel11@mail.ru
Svetlana Ju. Medvedeva
Institute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences
Cand. Sci. (Med.), Leading researcher
Russian Federation, 620049, Yekaterinburg, Pervomayskaya str., 106
Laboratoria.lab@yandex.ru
Arina Ju. Maksimova
Urals State Medical University
Junior researcher of the Central scientific research laboratory
Russian Federation, 620049, Yekaterinburg, Pervomayskaya str., 106
Laboratoria.lab@yandex.ru
Ekaterina V. Mandra
Urals State Medical University
Student
Russian Federation, 620049, Yekaterinburg, Pervomayskaya str., 106
jmandra@mail.ru
