Morphofunctional parameters of the liver after MMSC transplantation in animals with toxic liver damage

I.Yu. Maklakova¹,², V.V. Bazarnyi¹, D.Yu. Grebnev¹,², V.Ch. Vahrusheva¹

 

 

¹Ural State Medical University, Yekaterinburg, Russian Federation;

²Institute of Medical Cell Technologies», Yekaterinburg, Russian Federation

 

Abstract. Inflammation, as a typical pathological process, is the basis of many diseases. With the modern approach to the treatment of inflammatory diseases, cellular technologies are used. Multipotent mesenchymal stromal cells (MMSCs), having immunomodulatory properties, can be used to reduce inflammatory processes and restore the functional state of the liver in case of toxic damage. The aim of the study was to study the effect of MMSCs on changes in the morphofunctional state of the liver during its toxic damage. Materials and methods. In the study, MMSCs were transplanted in the amount of 4 million cells/kg of weight to mice under physiological conditions and under conditions of toxic liver damage. Toxic liver damage was modeled by introducing carbon tetrachloride (ССl₄) 50 µl. per mouse intraperitoneally, once. Assessment of liver morphometric parameters was performed on day 7 after modeling of liver pathology. The activity of DNA repair enzymes of the Poly-ADP-ribosopolymerase (PARP) family was also analyzed. Results. Transplantation of MMSCs to animals with toxic liver damage leads to an increase in the mitotic activity of hepatocytes, an increase in the number of binuclear hepatocytes, and an increase in the area of the nucleus. Also, the introduction of MMSC promotes the activation of DNA repair enzymes of the PARP family, which led to a decrease in programmed cell death and a decrease in the number of hepatocytes with micronuclei. Conclusion. In this study, it was shown that MMSC transplantation does not affect the physiological regeneration of the liver, but in case of toxic damage to the liver, it provides restoration of its morphofunctional state.

 

Keywords: placental multipotent mesenchymal stromal cells, liver regeneration, toxic liver damage

 

There is no conflict of interest.

Contact details of the corresponding author:

Dmitry Yu. Grebnev

dr-grebnev77@mail.ru

Received 26.11.2020

For citation:

Maklakova I.Yu., Bazarnyi V.V., Grebnev D.Yu., Vahrusheva V.Ch. Morphofunctional parameters of the liver after MMSC transplantation in animals with toxic liver damage. http://vestnikural.ru/article/1156 [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2020, Vol. 17, no. 4, pp. 340–346. DOI: 10.22138/2500-0918-2020-17-4-340-346 (In Russ)

 

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Authors

Irina Yu. Maklakova

Ural State Medical University

Candidate of Medical Sciences, Associate Professor of the Department of Pathological Physiology

3 Repin St., Yekaterinburg, Russian Federation, 620028

Institute of Medical Cell Technologies

Senior Researcher

22a Karl Marks str. Yekaterinburg Russian Federation, 620026

makliu@mail.ru

 

Vladimir V. Bazarnyi

Ural State Medical University

Doctor of Medical Sciences, Professor of the Department of Clinical Laboratory Diagnostics and Bacteriology

3 Repin St., Yekaterinburg, Russian Federation, 620028

vlad-bazarny@yandex.ru

 

Dmitry Yu. Grebnev

Ural State Medical University

Doctor of Medical Sciences, Head of the Department of Pathological Physiology

3 Repin St., Yekaterinburg, Russian Federation, 620028

Institute of Medical Cell Technologies

Senior Researcher

22a Karl Marks str. Yekaterinburg Russian Federation, 620026

dr-grebnev77@mail.ru

 

Victoria C. Vahrusheva

Ural State Medical University

Assistant at the Department of Pathological Physiology

3 Repin St., Yekaterinburg, Russian Federation, 620028

ezhik_13@inbox.ru

 
 
 

Authorization