MORPHOLOGICAL CHARACTERISTICS OF RATS SPLEEN WHEN EXPOSED TO THE NANOPARTICLES OF TITANIUM DIOXIDE
DOI: 10.22138/2500-0918-2018-15-6-830-839
L.A. Sharafutdinova, V.V. Valiullin
Bashkir State University, Ufa, Russian Federation;
Kazan Medical University, Kazan, Russian Federation
Abstract. Introduction. Titanium dioxide (TiO2) nanoparticles (NP) are widely used among other modern nanomaterials. They have a number of consumer advantages associated with good photocatalytic activity, high chemical and thermal stability and relatively low cost. However, a rapid increase in the number of publications dedicated to the toxic effects of TiO2-NP on various organs and tissues indicates a high level of interest among the researchers in the biological safety of their use. This is primarily due to the fact that the effects of TiO2-NP damage to the various cellular types of the body and the reasons of such vulnerability are practically not investigated. Objective. To study the morphofunctional changes in the rat spleens after long-term oral exposure to TiO2-NP. Material and methods. Classical morphological approaches and specific markers: proliferation — Ki- 67 and macrophages — CD68 were used in this study. Spleens of rats after daily oral administration (10 mg / kg body weight of the animal, 28 days) of TiO2-NP (rutile form, 40-60 nm) were investigated. Serial paraffin sections of the spleen were stained with hematoxylin and eosin. The immunohistochemical staining was performed using antibodies to Ki-67, CD68. Results. Morphometric analysis of the spleen tissues of the experimental group of animals revealed an increase in the area occupied by white pulp; an increase in the total number of lymphoid nodules, among which the share of primary lymph nodes considerably increased; a decrease in the absolute number of cells per unit area in the zones of white pulp compared with the similar indices of intact animals. The immunohistochemical study revealed a decrease in the intensity of cell proliferation in all compartments of the spleen and an increase in the number of macrophages in the red pulp in response to the effects of the nanoparticles under study. Conclusion. Oral administration of TiO2-NP lead to negative changes in the structural and immunohistochemical characteristics of the rat spleens, corresponding to the immunodepressive state.
Keywords: nanoparticles, titanium dioxide, spleen, Ki- 67, CD68
There is no conflict of interest.
Contact details of the corresponding author:
Lucia A. Sharafutdinova
sharafla@yandex.ru
Received 21.12.2018
For citation:
Sharafutdinova L.A., Valiullin V.V. Morphologi¬cal characteristics of rat spleens when exposed to titanium dioxide nanoparticles. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2018, Vol. 15, No. 6, pp. 830–839. DOI: 10.22138/2500-0918-2018-15-6-830-839 (In Russ).
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Authors:
Lucia A. Sharafutdinova
Cand. Sci. (Biolog.), Associate Professor of the Department of Physiology and General Biology, Faculty of Biology
Bashkir State University
Zaki Validi Str. 32 Ufa Russian Federation 450076 sharafla@yandex.ru
Viktor V. Valiullin
Dr. Sci. (Biolog.), Professor of the Department of
Histology
Kazan Medical University, Kazan, Russian Federation
Butlerova Str., 49 Kazan Russian Federation 420012 sharafla@yandex.ru
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)