MORPHOMETRIC ANALYSIS OF TISSUE STRUCTURES OF THE RAT GINGIVAL MUCOSA UNDER THE INFLUENCE OF INFRARED LASER RADIATION
DOI: 10.22138/2500-0918-2021-18-3-190-196
R.U. Giniatullin¹, E.R. Melnikova²
¹South Ural State Medical University, Chelyabinsk, Russian Federation;
²Set of dental clinics of Vita Smile LLC, Chelyabinsk, Russian Federation
Abstract. The aim of the study — was a comparative study of the adaptive responses of tissue structures and gelatinases of the gingival mucosa (SOD) in healthy rats to the effect of different modes of low-intensity laser radiation (NILI) in the infrared range. Materials and methods of research: the work was performed on 55 laboratory mature female rats using histological, immunohistochemical, zymographic, morphometric and statistical methods. The results of the study: the obtained data confirm the development of biological effects in response to the action of NILI: absorption of photon energy by intracellular components стим stimulation of adaptation reactions: increased activity of enzymes and TC function, activation of microcirculation. Conclusions: 1. After 1 minute and on the 1st day after exposure to NILI with a wavelength of 970 nm and a power of 0.5 W, a more significant increase in the number of TC with VEGFR1 expression, degranulated forms of TC, including with the 1st, 2nd and 3rd degrees of degranulation, the number, diameter of blood vessels and the area occupied by them, and an increase in gelatinase activity was recorded up to 5 days compared to similar indicators at the specified periods after exposure to NILI with a wavelength of 890 nm and a capacity of 25 MW. 2. The presence of a direct strong correlation between the parameters of TC, blood vessels and the activity of gelatinases indicates the development of interrelated physiological processes of adaptation in SOD tissues in response to the action of various parameters of the NILI infrared range. 3. The immunohistochemical method allows for specific detection, precise localization, and objective morphometric analysis of TC with VEGFR1 expression in SOD of control and experimental rats.
Keywords: rats, SOD, NILI, TC, VEGFR1, blood vessels, gelatinases
There is no conflict of interest.
Contact details of the corresponding author:
Ravil U. Giniatullin
prkom@chelsma.ru
Received 12.07.2021
For citation:
Giniatullin R.U., Melnikova E.R. Morphometric analysis of tissue structures of the rat gingival mucosa under the influence of infrared laser radiation. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2021, Vol. 18, no. 3, pp. 190–196. DOI: 10.22138/2500-0918-2021-18-3-190-196 (In Russ)
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Authors
Ravil U. Giniatullin
South Ural State Medical University, Department of Pathological Anatomy and Forensic Medicine
Doctor of Medical Sciences, Professor
64 Vorovsky str., Chelyabinsk Russian Federation 454092
prkom@chelsma.ru
Elena R. Melnikova
Set of dental clinics of Vita Smile LLC, Chelyabinsk, Russian Federation
Candidate of Medical Sciences, dentist-therapist
35 Br. Kashirinykh str., office 21 Chelyabinsk Russian Federation 454136
info@vitasmail.ru
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)