Disturbances of peripheral blood lymphocytes proliferation in schoolchildren living in rural areas of Murmansk Region

DOI: 10.22138/2500-0918-2019-16-2-223-229

V.V. Pozharskaya

Research Centre for Human Adaptation in the Arctic, Kola Science Centre, Russian Academy of Sciences, Apatity, Russian Federation

Abstract. Aim — to reveal the dependence of the violation of proliferation of peripheral blood lymphocytes in schoolchildren and the territoryliving. Materials and methods. Surveyed 58 schoolchildren. Comparison groups were formed from schoolchildren aged 15-16. The study design included interviewing, multi-parameter psychological testing, biochemical blood analysis and cytogenetic analysis of human peripheral blood lymphocytes. The micronucleus test was performed on stimulated lymphocytes of whole human peripheral blood in accordance with the standard method. Results and discussion. Studies have shown that the average frequencies of lymphocytes with micronucleus do not exceed the values established in the literature for comparison groups, but this indicator is close to the maximum values characteristic of the territory of Russia. General indicators of impaired lymphocyte proliferation indicate a higher proliferation rate among schoolchildren living in the Tersky district compared to other areas of the Murmansk region. Correlation analysis of the relationship of biochemical and immunological blood parameters with a number of cytogenetic disorders in school lymphocytes showed a significant relationship between the spontaneous frequency of occurrence of binucleated lymphocytes with the microkernel and immunoglobulin (lgE) in the blood. A significant correlation was found between the spontaneous frequency of lymphocytes with micronucleus, regardless of the ploidy of the cells, and the content of potassium in the blood.

Keywords: lymphocytes, schoolchildren, Аrctic

Received 19.04.2019.
For citation:
Pozharskaya V.V. Disturbances of peripheral blood lymphocytes proliferation in schoolchildren living in rural areas of Murmansk Region. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2019, Vol. 16, no. 2, pp. 223–229. DOI: 10.22138/2500-0918-2019-16-2-223-229 (In Russ)

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Author
Viktoria V. Pozharskaya
Research Centre for Human Adaptation in the Arctic, Kola Science Centre, Russian Academy of Sciences, Russian Federation
Cand. Sci. (Biol.), Researcher
Fersmana str. 41A Apatity Murmansk region Russian Federation 184209
vvp@medknc.ru

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