Neurotoxic Effects of the Titanium Dioxide Nanoparticles

DOI: 10.22138/2500-0918-2018-15-1-87-95

L.A. Sharafutdinova, A.M. Fedorova, S.A. Bashkatov, K.N.Sinelnikov, V.V. Valiullin

Bashkir State University, Ufa, Russian Federation;
Kazan Medical University, Kazan, Russian Federation

Abstract. Introduction. The intensive development of nanotechnology, the widespread use of nanoparticles (NP), many of which are not safe for organs and tissues of the body, can be considered as one of the potential threats leading to the development of various diseases. In view of the fact that NP are able to pass through hematopoietic barriers many of them are potentially dangerous for different CNS structures and can cause a variety of dysfunction of the central nervous system. The purpose of the study: to study the amplitude and spectral characteristics of the brain's electroencephalogram and the behavior of Wistar rats against the background of intranasal administration of the titanium dioxide NP (TiO2). Material and methods. Against the background of intranasal administration of NP TiO2, of the animals brain EEG was recorded with the help of monopolar needles on an 8-channel Neuron-Spectrum-1B electroencephalograph and the level of anxiety in rats was assessed using the «Elevated cross-shaped labyrinth» setup. Results. The study of the amplitude characteristics of the rats EEG showed that after 14 days of intranasal administration of NP TiO2 the average rhythms amplitude decreased both in the frontal and parietal regions of the cortex (p<0.05). A comparative analysis of the power spectra of the electroencephalogram made it possible to establish that by the 14th day of the experiment pronounced changes in the bioelectrical activity of the brains frontal cortex neurons are noted in the form of predominance of the slow wave activity of the θ and Δ bands due to a decrease in the relative power of the α, β1, β2 rhythms. The study of the behavioral peculiarities showed that intranasal administration of NP TiO2 causes an increase in the degree of anxiety in rats and in the level of research behavior. The conclusion. On the basis of the results of our study, we can conclude that intranasal management of NP TiO2 leads to a change in the amplitude and spectral characteristics of the brain's electroencephalogram and the behavior of the rats, which indicates their possible neurotoxic effect.

Keywords: neurotoxicity, nanoparticles, titanium dioxide, fontal cortex, electroencephalography, oscillations

Received 22.01.2018

For citation:
L.A. Sharafutdinova, A.M. Fedorova, S.A. Bashkatov, K.N.Sinelnikov, V.V. Valiullin. Neurotoxic Effects of the Titanium Dioxide Nanoparticles. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2018, Vol. 15, no. 1, pp. 87–95. DOI: 10.22138/2500-0918-2018-15-1-87-95 (In Russ)

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Authors
Lucia A. Sharafutdinova
Cand.Sci. (Bio), Associate Professor of the Department of Physiology and General Biology of the Faculty of Biology
sharafla@yandex.ru

Albina M. Fedorova
Cand.Sci. (Bio), Associate Professor of the Department of Physiology and General Biology of the Faculty of Biology
marmishka-06@mail.ru

Kirill N. Sinelnikov
Master of Biology Faculty
sikirax@gmail.com

Sergey A. Bashkatov
Dr. Sci. (Bio), Professor, Dean of Biological Faculty
s_bashkatov@list.ru

Bashkir State University
Russian Federation, 450076, Privolzhsky Federal District, Republic of Bashkortostan, Ufa, Zaki Validi Street 32

Viktor V. Valiullin
Kazan Medical University
Dr. Sci. (Bio), Professor of the Department of Histology
Doctor of Biological Sciences, professor of the Department of Histology
Russian Federation, 420012, Privolzhsky Federal District, Republic of Tatarstan, Kazan, Butlerova Street, 49 
valvv@kcn.ru