ASSESSMENT OF MORPHOFUNCTIONAL CHANGES OF THE LIVER AFTER ITS RESECTION ON THE BACKGROUND OF INTRODUCTION OF MULTIPOTENT MESENCHYMAL STROMAL CELLS IN AGING CONDITIONS
DOI: 10.22138/2500-0918-2020-17-1-89-97
V.Ch. Vahrusheva, I.Yu. Maklakova, D.Yu. Grebnev, V.V. Bazarnyi, I.V. Gavrilov
Ural State Medical University, Yekaterinburg, Russian Federation;
Institute of Medical Cell Technologies, Yekaterinburg, Russian Federation
Abstract. The aim of the study was to study the effect of multipotent mesenchymal stromal cell transplantation (MMSC) on liver regeneration under physiological conditions and after its resection under aging conditions. Materials and methods. Isolation of MMSC culture was carried out from the placenta chorion of 5 laboratory animal mice-females aged 3-4 months, weighing 22-23 g, gestation period of 18 days. MMSC was cultured in a CO2 incubator at a temperature of 37°C with a carbon dioxide content of 5% and a humidity of 90%. MMSCs of the third passage at a dose of 4 million cells/kg were used for transplantation to laboratory animals. MMSCs were administered under physiological conditions (without liver resection) and 1 hour after Subtotal liver resection to Mature and old laboratory animals. Biochemical indices of peripheral blood and liver morphometric indices were evaluated on 1, 3, 7 days after MMSC administration. Results. Transplantation of MMSCs to mature and old laboratory animals without liver resection does not lead to changes in biochemical parameters of peripheral blood and morphometric parameters of the liver. On day 3 after liver resection on the background of MMSC administration in mature laboratory animals, a decrease in the level of AST, ALT, alkaline phosphatase was found in comparison with the control subgroup of mature animals. Have old animals this effect emerges on 7 clock. In mature and old animals, an increase in the area of the nucleus of hepatocytes, nuclear cytoplasmic index (YCI), the number of binuclear cells was found. In mature laboratory animals revealed a decrease in apoptosis, increased mitotic activity, which led to an increase in the number of hepatocytes. At the same time, in older animals, there was a decrease in the apoptotic index, the level of mitosis, the number of hepatocytes did not differ from the data of the control subgroup. Conclusion. It was found that transplantation of MMSCs to mature and old laboratory animals after Subtotal resection of the liver stimulates its regeneration. In mature animals, activation of cellular regeneration (increase in the number of hepatocytes) and intracellular (increase in the number of binuclear cells, YCI) was revealed, whereas in old animals-only due to the activation of intracellular regeneration.
Keywords: multipotent mesenchymal stromal cells, liver regeneration, biochemical parameters, morphometric parameters
Received 10.12.2020
For citation: Vahrusheva V.Ch., Maklakova I.Yu., Grebnev D.Yu., Bazarnyi V.V., Gavrilov I.V.Assessment of morphofunctional changes of the liver after its resection on the background of introduction of multipotent mesenchymal stromal cells in aging conditions. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2020, Vol. 17, no. 1, pp. 89–97. DOI: 10.22138/2500-0918-2020-17-1-89-97 (In Russ)
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Authors
Victoria Ch. Vahrusheva
Ural State Medical University
Postgraduate of the Department of Pathological Physiology
3 Repin str. Yekaterinburg Russian Federation 620028
yusupova1@inbox.ru
Irina Yu. Maklakova
Ural State Medical University
Cand. Sci. (Med.), Associate Professor of the Department of pathological physiology
3 Repin str. Yekaterinburg Russian Federation 620028
Institute of Medical Cell Technologies
Senior researcher
22a Karl Marx str. Yekaterinburg Russian Federation 620026
makliu@mail.ru
Dmitry Yu. Grebnev
Ural State Medical University
Dr. Sci. (Med.), Head of the Department of pathological physiology
3 Repin str. Yekaterinburg Russian Federation 620028
Institute of Medical Cell Technologies
Senior researcher
22a Karl Marx str. Yekaterinburg Russian Federation 620026
dr-grebnev77@mail.ru
Vladimir V. Bazarnyi
Ural State Medical University
Dr. Sci. (Med.), Professor of the Department of clinical laboratory diagnostics and bacteriology,
3 Repin str. Yekaterinburg Russian Federation 620028
vlad-bazarny@yandex.ru
Ilya V. Gavrilov
Ural State Medical University
Cand. Sci. (Bio.), Associate Professor
3 Repin str. Yekaterinburg Russian Federation 620028
Institute of Medical Cell Technologies
Senior researcher
22a Karl Marx str. Yekaterinburg Russian Federation 620026
given18@yandex.ru