EVALUATION OF THE INFLUENCE OF HIGH-INTENSITY LASER OF DIFFERENT WAVELENGTHS ON SOME REGULATORY CELLULAR POPULATIONS OF NORMOTROPHIC SKIN SCAR
DOI: 10.22138/2500-0918-2020-17-1-72-79
M.I. Astakhova, L.V. Astakhova, E.S. Golovneva, T.G. Kravchenko
Multidisciplinary center for laser medicine, Chelyabinsk, Russian Federation;
South-Ural State Medical University, Chelyabinsk, Russian Federation
Abstract. The effect of high-intensity laser of different wavelengths on biological tissues differs in penetration depth and interaction with chromophores, which can affect the degree of tissue structure damage and functional activity of cells. The aim of the work was to assess the dynamics of the regulatory cells content in the normotrophic skin scar depending on the wavelength of high-intensity laser exposure. The experiment was carried out on 30 rats with a model of scars in the paravertebral regions. The effects of laser with wavelengths of 0.66 μm, 1.06 μm and 10.6 μm were studied. The dose and power density of laser energy in the experimental groups were comparable. Animals were removed from the experiment after 1 hour, 1, 3 and 7 days. The number of cells was analyzed in histological sections by morphometric method. The results of the study showed that 0.66 and 1.06 μm laser exposition increased the number and index of degranulation of mast cells from 1 hour to 3 days after; 10.6 μm laser activated mast cells starting from the 1st day in all layers of the scar. The number of lymphocytes increased on 1 day and 3 days after all laser expositions. The number of macrophages increased from 1 day after 1.06 μm laser irradiation, and on the 3 day for all other wavelengths. On 7 day, the number of macrophages increased only in the subepidermal layer of scars in the 10.6 μm laser group. Thus, the regulatory cells consistently increased their representation in the scar tissue after exposure to laser. Features of regulatory cell reactions on laser of different wavelengths may affect the activity of fibroblasts, collagen production and scar remodeling.
Keywords: laser, skin scar, mast cells, lymphocytes, macrophages
Received 10.12.2020
For citation: Astakhova M.I., Astakhova L.V., Golovneva E.S., Kravchenko T.G. Evaluation of the influence of high-intensity laser of different wavelengths on some regulatory cellular populations of normotrophic skin scar. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2020, Vol. 17, no. 1, pp. 72–79. DOI: 10.22138/2500-0918-2020-17-1-72-79 (In Russ).
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Authors
Marija I. Astakhova
Multidisciplinary center for laser medicine
Researcher
287 Pobedy av. Chelyabinsk, Russian Federation 454021
laser-chelyabinsk@yandex.ru
Ljudmila V. Astakhova
Multidisciplinary center for laser medicine
Cand. Sci. (Med.), Head of Research
287 Pobedy av. Chelyabinsk, Russian Federation 454021
bonikva@mail.ru
Elena S. Golovneva
SouthUral State Medical University
64 Vorovskogo str. Chelyabinsk Russian Federation 454092
Dr. Sci. (Med.), Professor of Department of the Normal Physiology
micron30@mail.ru
Tat'jana G. Kravchenko
Multidisciplinary center for laser medicine
Cand. Sci. (Bio.), Senior Researcher
287 Pobedy av. Chelyabinsk, Russian Federation 454021
bossanova@yandex.ru