Estimation of the efficiency of application of tissue engineering structure based on autological chondroblasts for osteoarthrosis therapy

A.V. Korotkov¹^,², S.V. Kostyukova¹^,², D.A. Sichkar¹^,²,

E.A. Shuman¹^,², O.G. Makeev¹^,²

¹Institute of Medical Cell Technologies, Yekaterinburg, Russian Federation;

²Ural State Medical University, Yekaterinburg, Russian Federation

Abstract. The aim of the work is to evaluate the effectiveness of the use of multipotent mesenchymal stromal cells (HMSC) differentiated into chondroblasts in the treatment of damage to the surface of the articular cartilage on the model of experimental mechanical damage to the articular cartilage.

The study involved twelve male Chinchilla rabbits. In the first group of animals, full-layer damage to the left and right distal cartilage of the knee joint was mechanically mechanical using trepan. The second group has non-full-layer damage to the corresponding joints. In the cavity of the right joints of experimental animals, 2×10⁶ HMSCs were injected in saline. In the cavity of the left joints, 0.5 ml of saline solution of sodium (control group). After 80 days from the time of the operation, an analysis of the macro and microscopic picture of the articular surfaces on the O’Driscoll scale was performed.

It was shown that intra-articular administration of HMSC has a pronounced preparation effect in relation to the cartilage tissue of the joint, with full-layer and non-full-layer injuries.

Keywords: knee joint, cartilage defect, chondroblasts

There is no conflict of interest.

Contact details of the corresponding author:

Oleg G. Makeev

larim@mail.ru

Received 26.11.2020г

For citation:

Korotkov A.V., Kostyukova S.V., Sichkar D.A., Shuman E.A., Makeev O.G. Estimation of the efficiency of application of tissue engineering structure based on autological chondroblasts for osteoarthrosis therapy. http://vestnikural.ru/article/1155 [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2020, Vol. 17, no. 4, pp. 333–339. DOI: 10.22138/2500-0918-2020-17-4-333-339 (In Russ)

REFERENCE

Salzmann G. M., Sah B. R., Schmal H., Niemeyer P., Sudkamp N. P. et al. Microfracture for treatment of knee cartilage defects in children and adolescents. Pediatric reports. 2012; 4. ( 2) :212-224
Roberts S., Menage J., Sandell L. J., Evans E. H., Richardson J. B. et al. Immunohistochemical study of collagen types I and II and procollagen IIA in human cartilage repair tissue following autologous chondrocyte implantation. The Knee. 2009; 16. (5): 398-404.
Park Y. G., Han S. B., Song S. J., Kim T. J., Ha C. W. et al. Plateletrich plasma therapy for knee joint problems: review of the literature, current practice and legal perspectives in Korea Knee surgery & related research. 2012; 24 (2): 70-78.
Mastrangelo A. N., Vavken P., Fleming B. C., Harrison S. L., Murray M. M. et al. Reduced platelet concentration does not harm PRP effectiveness for ACL repair in a porcine in vivo model. Journal of Orthopaedic Research. 2011; 29 (7): 1002-1007.
Everts P. A., Knape J. T., Weibrich G., Schönberger J. P., Hoffmann J., Overdevest E. P., van Zundert A. et al. Plateletrich plasma and platelet gel: a review. The Journal of extracorporeal technology. 2006; 38 (2): 174 187.
Orth P., Zurakowski D., Wincheringer D., Madry H. et al. Reliability, reproducibility, and validation of five major histological scoring systems for experimental articular cartilage repair in the rabbit model Tissue Engineering Part C: Methods. 2011; 18. (5): –329-339.
Kon E., Buda R., Filardo G., Di Martino A., Timoncini A., Cenacchi A., Marcacci M. et al. Plateletrich plasma: intraarticular knee injections produced favorable results on degenerative cartilage lesions Knee Surgery, Sports Traumatology, Arthroscopy. 2010; 18 (4): 472-479.
Redler L. H., Thompson S. A., Hsu S. H., Ahmad C. S., Levine W. N. et al. Plateletrich plasma therapy: a systematic literature review and evidence for clinical use. The Physician and sportsmedicine. 2011; 39 (1): 42-51

Authors

Svetlana V. Kostyukova