Oxytocin Reactivity of Erythrocytes in Pregnant Women and Parturient Women and the Impact on it of Atosiban and Dydrogesterone

DOI: 10.22138/2500-0918-2017-14-4-399-413

V.I Tsirkin, K.Yu. Anisimov, O.M. Bezmeltseva, E.N. Bushkova, O.A. Bratukhina, S.L. Dmitrieva, T.V. Cherepanova

Vyatka State University, Kirov, Russian Federation
Kazan State Medical University, Kazan, Russian Federation
Ural State Medical University, Ekaterinburg, Russian Federation
Institute of Physiology Komi Science Centre, Syktyvkar; Kirov State,
Kirov Regional Clinical Perinatal Center, Kirov, Russian Federation
Women's consultation No.9, Kirov, Russian Federation

Abstract. The purpose of the study was to investigate the oxytocinoreactivity of erythrocytes and the influence of dydrogesterone on it at women with uncomplicated pregnancy and labor as well as women with the threat of premature birth. Material and methods of investigation. The erythrocytes of heparinized venous blood of women with uncomplicated course of pregnancy (I, II and III trimesters), women with the threat of premature labor (TPL) and parturient women (I period) was investigate. The agglutination and sedimentation capacity of erythrocytes was evaluated under the influence of oxytocin (OT, 10-7–10-3 IU/ml), dydrogesterone (water-soluble progesterone analog, 10-8–10-5 g/ml), atosiban (oxytocin receptor blocker), as well as mixtures of oxytocin together with dydrogesterone (10-6 g/ml), or together with atosiban (10-6 g/ml). Agglutination ability was assessed by changing of the start time of agglutination (STA) of erythrocytes induced by salt extract of bean seeds, containing phytohemagglutinin (PHAb), and the sedimentation capacity was assessed according to the ESR by our modified Panchenkov method.Results of the study. Oxytocin (in one or another concentration) in all groups reduced STAPHAb, those accelerated of agglutination rate. Atosiban (10-10–10-6 g/ml) reduced the background STAPHAb in pregnant women (II, III) and parturient women, but did not change it at women with TPL, and at a concentration of 10-6 g/ml atosiban blocked the reduction of STAPHAb to oxytocin in pregnant women (I and II) and at woman with TPL, but increased the decrease of STAPHAb in parturient women. Dydrogesterone (10-6 g/ml) in all groups did not affect on the background STAPHAb but increased the response of erythrocytes to oxytocin at pregnant women (II), and reduced it in the remaining groups. When assessing ESR, it was shown that background values of ESR during pregnancy increase. In all five groups, low concentrations of oxytocin (10-7, 10-6 IU/ml) reduced ESR, and high concentrations (10-4, 10-3 IU/ml) either did not change ESR, or increased it. In all groups, dydrogesterone (10-6 g/ml) did not affect background ESR, but blocked the response of erythrocytes to low concentrations of oxytocin (except for pregnant women II) and changed the nature of the response to high concentrations of oxytocin (instead of increasing ESR oxytocin reduced it).Conclusions. At pregnancy, labor and at TPL the nature of the response of erythrocytes to oxytocin, progesterone and atosiban changes. Progesterone is able non genomic changing the effectiveness of OTP activation — reducing it during pregnancy and increasing at labor. This reveals yet another mechanism of action of progesterone as the main hormone, which provides optimal contractile activity of the uterus when fetus is born. The results of experiments with atosiban indicate heterogeneity of oxytocin receptors in erythrocytes and give a basis for its separating atosiban-bloking receptors (OTRAB) and atosiban-nonblocking receptors (OTRANВ) or atosiban-activated receptors (OTRAA) OTRAВ have a high sensitivity to OT, and their activation reduces ESR. Conversely, OTRANВ (or OTRAA) has a low sensitivity to OT, and their activation increases ESR. At pregnancy in erythrocytes the OTRAB is mainly revealed, and in labor revealed OTRANB. The results of the study can be used in clinical practice when assessing the state of the mechanisms of regulation of uterine contractile activity.

Keywords: pregnancy, labor, the threat of premature labor, erythrocytes, agglutination, sedimentation, oxytocin, atosiban, progesterone

Received 31.08.2017

For citation:
Tsirkin V.I., Anisimov K.Yu., Bezmeltseva O.M., Bushkova E.N., Bratukhina O.A., Dmitrieva S.L. Cherepanova T.V. Oxytocin reactivity of erythrocytes in pregnant women and parturient women and the impact on it of atosiban and dydrogesterone. Vestn. Ural. Med. Akad. Nauki. Journal of Ural Medical Academic Science. 2017, Vol. 14, no. 4, pp. 399–413. DOI: 10.22138/2500-0918-2017-14-4-399-413 (In Russ)

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Authors
Victor I. Tsirkin
Kazan State Medical University
Dr. Sci. (Med.), Professor, Department of Normal Physiology
Russian Federation, 420002, Kazan, ul. Butlerova, 49
Institute of Biology and Biotechnology, Vyatka State University
Russian Federation, 610000, Kirov, ul. Moskovskaja, 36
tsirkin@list.ru

Konstantin Yu. Anisimov
Ural State Medical University
Cand. Sci. (Med.), Assistant, Department of Obstetrics and Gynecology
Russian Federation, 620028, Yekaterinburg, ul. Repina, 3
kuanisimov@mail.ru

Oksana M. Besmeltseva
Vyatka State University
Graduate student of the Institute of Biology and Biotechnology
Russian Federation, 610000, Kirov, 36 Moskovsky St.
Institute of Physiology of the Komi Scientific Center of the Ural Branch of the Russian Academy of Sciences
Junior researcher at the Laboratory of Blood Cryophysiology
Russian Federation, Kirov, Lenina str., 168a
Oksana_bezmeltseva@mail.ru

Elena N. Buskova
Vyatka State University
Physiologist of the Department of Biology and Methods of Teaching of Biology
Russian Federation, 610000, Kirov, 36 Moskovsky St.
elena_bushkova@mail.ru

Olga A. Bratuhina
Kirov regional clinical perinatal center
Cand. Sci. (Med.), Head of Obstetrics department of pregnancy pathology № 1 
Russian Federation, 610048, Kirov, Moscow street, 163
mail@pncenter.ru

Svetlana L. Dmitrieva
Kirov regional clinical perinatal center
Cand. Sci. (Med.), Head of Postpartum department
Russian Federation, 610048, Kirov, Moscow street, 163
Swdmitr09@yandex.ru

Tatiana V. Cherepanova
Women's consultation No. 9
Russian Federation, 610001, Kirov, Nekrasov st, 6A
gb9@medkirov.ru