FEATURES OF THE CYTOKINE PROFILE IN BURN PATIENTS
DOI: 10.22138/2500-0918-2020-17-2-161-174
P.A. Selivanov, N.V. Bychkova, N.V. Chinenova, T.G. Khabirova, N.M. Kalinina
Nikiforov Russian Center of Emergency and Radiation Medicine, EMERCOM of Russia, St. Petersburg, Russian Federation
First Pavlov State Medical University of St. Petersburg, St. Petersburg, Russian Federation
3 Military hospital of the National Guard of the Russian Federation, St. Petersburg, Russian Federation
St. Petersburg I. I. Dzhanelidze Research Institute of Emergency Medicine, St. Petersburg, Russian Federation
Abstract. Introduction. Currently, more and more attention is paid to burn injury as one of the most significant medical and social problems. Treatment of burn injury is performed by autotransplantation of the skin flap. The efficiency of using this method is from 70 to 90%. A significant reason for the failure of skin flap transplantation in burn disease is the lack of objective methods for assessing the patient's condition on the eve of free autodermoplasty surgery. Assessment of the levels of both Pro-and anti-inflammatory cytokines in burned patients is not included in the algorithm of examination and monitoring of the status of these patients. The aim of the study: to study the levels of pro — and anti-inflammatory cytokines to expand the algorithm of laboratory examination in preparing patients for free autodermoplasty.Materials and methods. The levels of IL-6, IL-8, and IL-10 in the peripheral blood of 50 patients with burn injury compared to healthy individuals were studied by enzyme immunoassay. Results. The serum content of all studied cytokines was significantly increased upon admission to the hospital in all burn patients compared to healthy individuals. A particularly pronounced increase was observed in patients with a fatal outcome. The results of autodermoplasty correlated with the severity of hyperproduction of proinflammatory cytokines on the eve of surgery. Conclusion. A justification for the addition of the algorithm for laboratory examination of burned patients by determining cytokines-interleukins 6, 8 and 10. Monitoring the level of cytokines in burn injury is appropriate for objectifying the choice of the timing of surgery, which will help reduce the mortality rate and increase the effectiveness of free autodermoplasty.
Keywords: cytokines; burns; autodermoplasty
Received 15.07.2020
For citation:
Selivanov P.A., Bychkova N.V., Chinenova L.V., Khabirova T.G., Kalinina N.M. Features of the cytokine profile in burn patients. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2020, Vol. 17, no. 2, pp. 161–174. DOI: 10.22138/2500-0918-2020-17-2-161-174 (In Russ)
REFERENCES
1. Petrovskaja O.N., Rimzha M.I., Zolotukhina R.V. Social and hygienic factors of burn injuries in adults. Medical journal BSMU = Medicinskijj zhurnal BGMU. 2016; 3(32): 99-102. (In Russian)
2. Bozhedomov A.Yu., Morrison V.V., Shulaeva N.M., Nikitina V.V., Ivanenko I.L. Cytoprotective effect of cytoflavin in the treatment of thermal injury of varying severity. Saratov Journal of Medical Scientific Research = Saratovskij nauchno-medicinskij zhurnal. 2012; 8(1): 38–42. (In Russian)
3. Burmeister D.M., McIntyre M.K., Baker B.A., Rizzo J.A., Brown A., Natesan S., et al. Impact of Isolated Burns on Major Organs: A Large Animal Model Characterized. Shock. 2016; 46(3): 137-147. doi: 10.1097/SHK. 0000000000000662.
4. Malyutina N. B. Rational use of methods of early surgical treatment of deep burns in patients of older age groups. Combustiology = Kombustiologiya. 2002; 10. Available at: http://combustiolog.ru/journal/ratsional-noe-primenenie-metodov-rannego-hirurgicheskogo-lecheniya-glubokih-ozhogov-u-patsientov-starshih-vozrastny-h-grupp/ (accessed 25 March 2020). (In Russian)
5. Usov V.V., Oby`dennikova T.N., Tarasov A.E., Gorsheev A.N. Assessment of the immune status of severely burned patients. Pacific Medical Journal = Tikhookean. med. Zhurn. 2008;1: 53–55. (In Russian)
6. Konenkov V.I. Dynamics of changes in the concentration of cytokines and neutrophil functions in the blood of rats after a thermal burn. Cytokines and inflammation = Citokiny` i vospalenie. 2007; 6(3): 57-61. (In Russian)
7. Ivanenko I.L., Gladilin G.P., Nikitina V.V., Veretennikov S.I. The role of cytokines in the pathogenesis of complications in burn disease. Fundamental research = Fundamental`ny`e issledovaniya. 2015;1-4: 752-754. (In Russian)
8. Potapnev M.P., Hushchyna L.M., Moroz L.А. Human neutrophils subpopulations and functions heterogeneity in norm and pathology. 2019; 40 (5): 84–96. doi: 10.24411/0206-4952-2019-15009.
9. Simbircev A.S., Gromova A.Yu Functional polymorphism of genes of inflammatory regulatory molecules. Cytokines and inflammation = Citokiny` i vospalenie. 2005; 4(1): 3-10. (In Russian)
10. WMA Declaration of Helsinki — Ethical Principles for Medical Research Involving Human Subjects. 2013; 310: 2191-2194.
11. Yudina T.V., Saarkoppel` L.M., Kryuchkova E.N., Konovalov I.M., Mirzonov V.A. Determination of cytokine balance in assessing the health status of industrial workers. Guidelines = Metodicheskie rekomendacii MR 2.2.9.0049-11. 2011; 1-18. (In Russian)
12. Morrison V.V., Bozhedomov A.Yu. Dynamics of indicators of hemostasis and endothelial dysfunction in thermal trauma. Bulletin of RSMU = Vestnik RGMU. 2019; 2: 72-76. (In Russian)
13. Krivoshapka A.V., Berezhnaya A.V., Mironchenko S.I. Effect of methyluracyl ointment on the level of proinflammatory cytokines in burn injury in rats. European Student Scientific Journal = Evropejskij studencheskij nauchny`j zhurnal. 2013;2. Available at: http://repo.knmu.edu.ua/handle/123456789/4425 (accessed 10 april 2020). (In Russian)
14. Zvyagin A.A., Demidova V.S., Smirnov G.V. Biological markers in the diagnosis and treatment of sepsis (literature review). Wounds and wound infections. The prof. B.M. Kostyuchenok journal = Rany` i ranevy`e infekcii. Zhurnal imeni professora B.M. Kostyuchyonka. 2016; 19-23. (In Russian)
15. Jun Hur, Hyeong Tae Yang, Wook Chun, Jong-Hyun Kim. Inflammatory cytokines and their prognostic ability in cases of major burn injury. Ann Lab Med.2015; 35: 105-110.
Authors
Petr A. Selivanov
Nikiforov Russian Center of Emergency and Radiation Medicine, EMERCOM of Russia
PhD student (Medicine), Department of therapy and integrative medicine
4/2 Academica Lebedeva Str., St. Petersburg, 194044, Russian Federation
olliwooood@gmail.com
Natalia V. Bychkova
Nikiforov Russian Center of Emergency and Radiation Medicine, EMERCOM of Russia,
PhD (Biology), senior researcher, Laboratory of Clinical Immunology
4/2 Academica Lebedeva Str., St. Petersburg, 194044, Russian Federation
BNV19692007@yandex.ru
Lyudmila V. Chinenova
Nikiforov Russian Center of Emergency and Radiation Medicine, EMERCOM of Russia,
PhD (Medicine), clinical laboratory specialist of the Laboratory of Clinical Immunology
4/2 Academica Lebedeva Str., St. Petersburg, 194044, Russian Federation
Tatyana G. Khabirova
I.I. Dzhanelidze St. Petersburg research Institute
head of the laboratory of immunology
St. Petersburg, 3 Budapest street, lit. A, 192242, Russian Federation
tagira_86@mail.ru
Natalia M. Kalinina
Nikiforov Russian Center of Emergency and Radiation Medicine, EMERCOM of Russia
Dr (Medicine), Professor, chief researcher of the Department of Laboratory Diagnostics
4/2 Academica Lebedeva Str., St. Petersburg, 194044, Russia,
doct.kalin@mail.ru