Immunogenetic status peculiarities of children's population in the nitrate hydrogeological province conditions of Perm Region
DOI: 10.22138/2500-0918-2019-16-2-209-214
O.V. Dolgikh, I.N. Alikina, Y.A. Chelakova, O.A. Kazakova
FBSI «Federal Scientific Center for Medical and Preventive Health Risk Management Technologies», Perm, Russian Federation
Abstract: The aim of the work is to study the child population immunogenetic status peculiarities in the conditions of nitrate hydrogeological province of Perm Krai. Materials and methods. Cell differentiation markers (CD3+CD95+) were determined by flow cytometry on FACSCalibur Flow cytometer (Becton Dickinson company), using CellQuestPrO universal software. Expression level determination of proteins controlling apoptosis — Bcl-2, p53 was performed using the appropriate monoclonal antibodies («BC», USA) and simultaneous negative isotypic control procedure. Genetic features were detected by real-time polymerase chain reaction and allele discrimination based on the diagnosis of single-nucleotide polymorphisms. Results. A significant increase in specific IgG to N-nitrosodimethylamine (p<0.05) was found, both in relation to the reference value and in relation to the comparison group. Changes in cellular immunity (inhibition of t-cell receptors CD3+CD95+ and intracellular proteins Bcl-2), increased expression of tumor markers associated with an increase in the concentration of N-nitrosodimethylamine in the blood were found to be authentic both in relation to the reference level and the comparison group. The results of studying the frequency of polymorphisms revealed its peculiarities in children nitrate province according to the criterion of a minor allele of methylenetetrahydrofolate reductase gene MTHFR, and genes of proliferation peroxisome PPARA genes detoxification coproporphyrinogen (CPOX) and cytochrome (CYP1A2), ecopolitical gene (BRCA1), characterizing the probability of occurrence of violations of the functions of detoxification and ecopolitical. There was a significant (p=0.016) increase in the minor allele of the enzyme BRCA1 rs3950989 gene the occurrence frequency associated with a deficiency of tumor necrosis factor (TNFR), which indicates the formation of additional risk factors in the technogenic geohydrochemical province conditions, which may be associated with carcinogenesis. Findings. Immune reactivity and genetic polymorphism violations indicate their characteristics in the population of nitrate technogenic chemical province of Perm Krai, which characterize and can be used as markers of the pathological health disorders formation associated with tumor-associated processes and cellular immunodeficiency, which implementation can contribute to the identified imbalance of cellular and humoral immunity (deficiency of proapoptotic factors and hyperproduction of IgG to nitrosamines).
Keywords: сell-mediated immunity, a genetic polymorphism, BRCA1 rs3950989 gene, nitrosamines
Received 15.04.2019г.
For citation:
Dolgikh O.V., AlikinaI.N., Chelakova Y.A., Kazakova O.A. Immunogenetic status peculiarities of children's population in the nitrate hydrogeological province conditions of Perm Region. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2019, Vol. 16, no. 2, pp. 209–214. DOI: 10.22138/2500-0918-2019-16-2-209-214 (In Russ)
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Authors
Inga N. Alikina
Junior researcher of the Department of immunobiological diagnostic methods
oleg@fcrisk.ru
Yulia A. Chelakova
Junior researcher of the Department of immunobiological diagnostic methods
oleg@fcrisk.ru
Oleg V. Dolgikh
Dr. Sci. (Med.), Head of the Department of immunobiological diagnostic methods
oleg@fcrisk.ru
Olga A. Kazakova
Junior researcher of the Department of immunobiological diagnostic methods
oleg@fcrisk.ru
FBSI «FSC for Medical and Preventive Health Risk Management Technologies»
Monastyrskaya str. 82 Perm Russian Federation 614045
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