FEATURES OF PROLIFERATIVE PROCESSES IN CELL CULTURES FROM SAMPLES OF LUMINAL B (HER2- NEGATIVE) SUBTYPE BREAST CARCINOMA
DOI: 10.22138/2500-0918-2023-20-1-53-62
E.V. Grebenyuk², A.S. Mogilenskikh¹, S.V. Sazonov¹,²
¹ Federal State Budgetary Educational Institution of Higher Education Ural State Medical University of the Ministry of Health of the Russian Federation,
Yekaterinburg, Russian Federation;
² State Autonomous Institution of Healthcare of the Sverdlovsk Region «Center for Specialized Types of Medical Care» Institute of Medical Cellular Technologies,
Yekaterinburg, Russian Federation
Abstract. Introduction. The Ki-67 cell proliferation marker is an indispensable point of the present morphological diagnosis of breast carcinoma, its association with the degree of malignancy and the recurrence risk for the disease has been substantiated. However dynamics of the Ki-67 index in cell culture from tumors of this subtype has not been studied enough. The aim of the study. To evaluate Ki-67 expression levels in cell cultures from samples of luminal B (HER2-negative) biological subtype tumors. Materials and methods. Tumor cells were extracted from BC samples by enzymatic method according to the standard protocol for obtaining a cell culture. A portion of cells from each passage was seeded on glasses in Petri dishes for immunocytochemical (ICC) analysis. ICC staining of the cells was conducted in autostainer Ventana (USA) using anti-Pan Keratin (AE1/AE3/PCK26) Primary Antibody (Roche diagnostics, the USA) and Ki-67 (clone of MIB-1, Dako, Denmark) antibodies. Results and discussion. Based on immunohistochemical (IHC) analysis of paraffin blocks the samples were determined as luminal B (Her2-negative) subtype. Cultures were obtained and the Ki-67 biomarker was determined at five passages. Significant differences in the values of the proliferation index between the examined passages were found. The epithelial phenotype of the tumor cells was saved as confirmed by the pancytoceratin staining data. Changes of proliferative activity showed the highest values of the biomarker at the first and third passages. The proliferation Ki-67 index value in the culture cells corresponded to the Ki-67 level in the tumor tissue of the luminal B (Her2-negative) subtype primary samples at the second, third and fourth passages. Conclusion. The high level of proliferative processes in the cell population maintaining the epithelial phenotype during cultivation allows the use of cell cultures as a comparative model for analyzing the expression of molecular biological markers and their compliance with primary cancer samples IHC data within a certain subtype.
Keywords: breast carcinoma, primary cell culture, Luminal B (HER2-negative) subtype, Immunocytochemistry
There is no conflict of interest.
Contact details of the corresponding author:
Ekaterina V. Grebenyuk
ev.grebenyuk9@yandex.ru
Received 13.04.2023
For citation:
Grebenyuk E.V., Mogilenskikh A.S., Sazonov S.V. Features of proliferative processes in Cell cultures from samples of luminal B (HER2-negative) subtype breast carcinoma. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2023, Vol. 20, no. 1-2, pp. 53–62. DOI: 10.22138/2500-0918-2023-20-1-53-62 (In Russ)
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Authors
Ekaterina V. Grebenyuk
Federal State Budgetary Educational Institution of Higher Education Ural State Medical University of the Ministry of Health of the Russian Federation
Postgraduate student of the 3rd year of study, specialty Pathological Physiology
State Autonomous Institution of Healthcare of the Sverdlovsk Region «Center for Specialized Types of Medical Care» Institute of Medical Cellular Technologies»
Researcher
ev.grebenyuk9@yandex.ru
Yekaterinburg, Russian Federation
Anna S. Mogilenskikh
Federal State Budgetary Educational Institution of Higher Education Ural State Medical University of the Ministry of Health of the Russian Federation
Researcher
State Autonomous Institution of Healthcare of the Sverdlovsk Region «Center for Specialized Types of Medical Care» Institute of Medical Cellular Technologies»
Researcher
annasajler@yandex.ru
Yekaterinburg, Russian Federation
Sergey V. Sazonov
Federal State Budgetary Educational Institution of Higher Education Ural State Medical University of the Ministry of Health of the Russian Federation
Head of the Department of Histology, Doctor of Medical Sciences, Professor
State Autonomous Institution of Healthcare of the Sverdlovsk Region «Center for Specialized Types of Medical Care» Institute of Medical Cellular Technologies»
Deputy Chief Physician for Science
prof-ssazonov@yandex.ru
Yekaterinburg, Russian Federation