SPECIFIC DISTRIBUTION OF HUMAN PAPILLOMAVIRUS TYPE IN PATIENTS OF DIFFERENT REPRODUCTIVE AGE WITH LOW AND HIGH-GRADE SQUAMOUS INTRAEPITHELIAL LESION

DOI: 10.22138/2500-0918-2021-18-4-282-294

M.I. Pakharukova¹, Yu.G. Lagereva¹,²., I.G. Starodubova¹, Ya.B. Beikin¹,²

¹Clinical and Diagnostic Center of Yekaterinburg, Yekaterinburg, Russian Federation;

²Science Institute of Immunology and Physiology, Ural Department of the Russian Academy of Sciences, Yekaterinburg, Russian Federation

Abstract. The purpose of this work was to study the characteristics of human papillomaviruses spread in reproductive age patients with low and high degree squamous intraepithelial lesions of the cervix in Yekaterinburg. Material and Methods. In with study 140 female patients of reproductive age with histologically confirmed low and high grade intraepithelial lesions were retrospectively included. HPV-genotyping by polymerase chain reaction was carried out in combination with liquid-based cytology BDSurePath. Formed 4 groups: LSIL, age from 18 to 30 years; LSIL, age from 31 to 44; HSIL, ages 18 to 30; HSIL, ages 31 to 44. Results. Analysis of the obtained data demonstrated that HPV 16 prevails in intraepithelial squamous cell lesions of the cervix of low and high degree in women of reproductive age in Yekaterinburg. In women aged 18-30 years, compared with the older reproductive age (31-44 years), multiple infections with 3, 4 or more HPV genotypes are more often recorded, regardless of the degree of damage. In addition to HPV 16, low-grade lesions in young women are most often associated with HPV 31, 51, and 52, and high-grade lesions with types 31, 33 and 52. HPV 33 is characteristic of a high degree of intraepithelial squamous cell lesions, especially in patients of older reproductive age. With intraepithelial squamous lesions of low and high degree, regardless of age, an equally high concentration of HPV DNA is observed.

Keywords: human papillomavirus, squamous intraepithelial lesions, type specific

There is no conflict of interest.

Contact details of the corresponding author: Pakharukova M.I. Pakharukovami@gmail.com

Received 08.10.2021

For citation: Pakharukova M.I., Lagereva Yu.G., Starodubova I.G., Beikin Ya.B. Specific distribution of human papillomavirus type in patients of different reproductive age with low and high-grade squamous intraepithelial lesion. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2021, Vol. 18, no. 4, pp. 282–294. DOI: 10.22138/2500-0918-2021-18-4-282-294 (In Russ)

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Authors:

Mariya I. Pakharukova

Clinical and Diagnostic Center of Yekaterinburg Biologist of the cytologycal laboratory 78-B 8 Marta st. Yekaterinburg Russian Federation 620144 Pakharukovami@gmail.com SPIN-код: 3698-8240, https://orcid.org/0000-0001-6019-3463

Irina G. Starodubova

Clinical and Diagnostic Center of Yekaterinburg Head of laboratory of molecular biological research methods 78-B 8 Marta st. Yekaterinburg Russian Federation 620144 snn@kdc-lab.ru

Yulia G. Lagereva

MD, head of laboratory of clinical immunology Clinical and Diagnostic Center of Yekaterinburg 78-B 8 Marta st. Yekaterinburg Russian Federation 620144 Senior researcher of laboratory of immunopathophysiology Science Institute of Immunology and Physiology, Ural Department of the Russian Academy of Sciences 106 st. Pervomaiskaya Yekaterinburg Russian Federation 620049 Anna-lagereva@yandex.ru Author ID: 568784, https://orcid.org/0000-0001-8211-0753

Yakov B. Beikin

MD, PhD, Professor, Head of Сlinical Diagnostic Center Clinical and Diagnostic Center of Yekaterinburg 78-B 8 Marta st. Yekaterinburg Russian Federation 620144 Head of laboratory of immunopathophysiology Science Institute of Immunology and Physiology, Ural Department of the Russian Academy of Sciences 106 st. Pervomaiskaya Yekaterinburg Russian Federation 620049 inbox@kdc-lab.ru Author ID: 271586

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