Application of Oligopeptides as Cytoprotectors at Accelerated Aging of Blood Cells IN VITRO

V.N. Meshchaninov, I.V. Gavrilov, V.A. Lukash, T.Yu. Verzhbitskaya,

D.A. Sentabreva, D.L. Shcherbakov, G.V. Zhiborkin

DOI: 10.22138/2500-0918-2017-14-4-355-361

Institute Medical Cell Technologies, Yekaterinburg, Russian Federation;
Ural State Medical University, Yekaterinburg, Russian Federation

Abstract. The length of storage of donor blood and the shaped elements, harvested for the purpose of subsequent blood transfusions, is limited. To increase the efficiency of blood storage, three targeted cell-oriented substances were selected: tripeptides glu-asp-arg: «pinealon», lys-glup-asp: «vesugene», pro-glup-asp «cryogen». These substances normalize metabolism and inhibit the aging of certain types of cells in the body of both healthy people and pathology. The aim of the study was to reveal the influence of oligopeptides of pinealon (glu-asp-arg), vesugene (lys-glup-asp) and crysgene (pro-glup-asp) on the cellular and biochemical composition of donor blood during accelerated aging of blood cells under in vitro storage conditions. Materials and methods. Whole blood and plasma of healthy people aged 30–59 years of both sexes were incubated with pinealon, vesugene and cryogen at a final concentration of 20 μg / ml. incubation 24 hours (early) and 48–72 hours (late). Blood plasma was used to study the basic parameters of carbohydrate, lipid and protein metabolism, chemiluminescence, whole blood — to study the resistance of erythrocytes, shaped elements, stem cells. Evaluation of the changes in the properties of erythrocyte membranes was carried out by a technique for studying the osmotic and peroxidative resistance of erythrocytes. The study was carried out at a high methodical level, taking into account the requirements of evidence-based medicine, modern equipment and appropriate reagents to it were used. The results were processed using nonparametric statistics. Results and conclusions. The data of the effect of oligopeptides of pinenealon (glu-asp-arg), vezugene (lys-glup-asp) and crysgene (pro-glup-asp) on the cellular and biochemical composition of donor blood are presented in the article. The inhibitory glycolysis in aging blood cells was revealed during hypoxic incubation in vitro, the action of oligopeptides of pinenealon (glu-asp-arg), vesugene (lys-glup-asp) and crysgene (pro-glup-asp), as well as a tendency to decrease chemiluminescence at a pronounced increase their (peroxide) resistance to oxidative stress. The investigated substances are presumably accelerated in the conditions of hypoxia maturation of blood stem cells. The content of CD34 + cells during storage of blood is subject to complex dynamics, in which, apparently, the transformation of stem cells into progenitor cells dominates. A conclusion is made about the effectiveness of their use as antioxidants, membrane protectors, cytogeuroprotectors.

Keywords: oligopeptides, blood, metabolism, conservation

Received 20.11.2017

For citation:
Meshchaninov V.N., Gavrilov V.N., Lukash V.A., Verzhbitskaya T.Yu., Sentabreva D.A., Shcherbakov D.L., Zhiborkin G.V. Application of oligopeptides as cytoprotectors at accelerated aging of blood cells in vitro. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2017, Vol. 14, no. 4, pp. 355–361. DOI: 10.22138/2500-0918-2017-14-4-355-361 (In Russ)

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Authors
Victor N. Meshchaninov
Institute Medical Cell Technologies;
Ural State Medical University
Dr. Sci. (Med.), Pofessor
22a, Karla Marksa st., Yekaterinburg, Russian Federation, 620026
mv-02@yandex.ru

Iliya V. Gavrilov
Institute Medical Cell Technologies;
Ural State Medical University
Cand. Sci. (Bio)
22a, Karla Marksa st., Yekaterinburg, Russian Federation, 620026
given18@yandex.ru

Vyacheslav A. Lucas
Institute Medical Cell Technologies;
Ural State Medical University
Cand. Sci. (Bio)
22a, Karla Marksa st., Yekaterinburg, Russian Federation, 620026
kafedra.biohimii@yandex.ru

Tatiana Yu. Verzhbitskaya
Institute Medical Cell Technologies
Cand. Sci. (Med.)
22a, Karla Marksa st., Yekaterinburg, Russian Federation, 620026
uralverba@gmail.com

Darya A. Sentabreva
Ural State Medical University
student, medical-prophylactic department
3, Repina st., Yekaterinburg, Russian Federation, 620028
darksaid-cosplay@yandex.ru

Denis L.Shcherbakov
Institute Medical Cell Technologies
Cand. Sci. (Bio)
22a, Karla Marksa st., Yekaterinburg, Russian Federation, 620026
cdcom2@yandex.ru

Gleb V.Zhiborkin
Institute Medical Cell Technologies
ophthalmologist
22a, Karla Marksa st., Yekaterinburg, Russian Federation, 620026
gleb1616@weburg.me