THE NATURE OF INCREASING OF THE ERYTHROCYTE SEDIMENTATION RATE IN PREGNANT WOMEN

DOI: 10.22138/2500-0918-2018-15-5-711-723

V.I. Tsirkin, К.Yu. Anisimov, E.G. Shushkanova, A.G. Kolpakov, E.E. Dushina, E.N. Bushkova, M.V. Bysheva, Т.V. Cherepanova

Vyatka State University, Kirov, Russian Federation;
Kazan State Medical University, Kazan, Russian Federation;
Ural State Medical University, Yekaterinburg, Russian Federation;
Women's consultation No9, Kirov, Russian Federation

Abstract. The aim of the work is to assess the role of the Ca-activated potassium channels (Gardos channels ) and sex hormones in increasing the erythrocyte sedimentation rate (ESR) in women during pregnancy. Material and methods of investigation. Heparinized venous blood of 13 women with uncomplicated pregnancy (7-37 weeks) and heparinized blood (from the heart) of 10 nonpregnant (phase of metaestrus) and 4 pregnant (5,7,7 and 21 d) rats were studied. ESR was determined by a modified method of Panchenkov (without sodium citrate). Methodological methods were used to reduce the activity of the Gardos channels of erythrocytes, including by binding Ca2+ ions to sodium citrate (290 mM), or by inhibiting the input of Ca2+ ions by verapamil (10-10-10-5 g/ml), or by blocking the Gardos channels with clotrimazole (10-10-10-4 g/ml) or hyperpotassium Krebs solution (10-60 mM KCl). Results of the study. ESR in women was 40 (32; 41) mm /hour. ESR was statistically significantly decreased under the influence of sodium citrate (up to 77% of background), verapamil, 10-8 g/ml (up to 57%), clotrimazole, 10-10-10-5 g/ml (up to 87-51%) or hyperpotassium solution, 10-60 mM (up to 79-51%.) The ESR in nonpregnant rats did not exceed 1-2 mm/hr and did not change under the influence of oxytocin (10-7-10-3 MI/ml), adrenaline (10-6 g/ml) and acetylcholine (10-6 g/ml). During pregnancy, ESR in rats did not increase, but even decreased (up to 1 mm/h) and did not change by dydrogesterone (10-6 g/ml). Conclusions The increase of ESR in pregnant women is due to the activation of the Gardos channels. Taking into account the data previously received by the authors that the ESR does not increase in pregnant women under the influence of dydrogesterone or esradiol valerate, and also, given the lack of an increase in ESR in rats during pregnancy, it is concluded that progesterone, and probably also estrogens, does not have a direct relationship to the increase in activity of the Gardos channels in pregnancy in women, and this function is most likely performed by the endogenous inhibitor of uterine myocyte contractility (EIUMC), whose expression probably increases in women with pregnancy under the influence of progesterone.It is postulated that the magnitude of the ESR of the venous heparinized blood of pregnant women and its change under the influence of various biologically active substances and effects can serve as important information reflecting the state of the system of regulation of uterine contractile activity.

Keywords: erythrocyte, Gardos channels, erythrocyte sedimentation rate, sodium citrate, verapamil, clotrimazole, pregnancy, rat, progesterone, estrogens

There is no conflict of interest.

Contact information of the author responsible for correspondence:
Victor I. Tsirkin
tsirkin@list.ru

Received 12.03.2018

For citation:
Tsirkin V.I., Anisimov К.Yu., Shushkanova E.G., Kolpakov A.G., Dushina E.E., Bushkova E.N., Bysheva M.V., Cherepanova Т.V. The nature of increasing of the erythrocyte sedimentation rate in pregnant women. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2018, Vol. 15, no. 5, pp. 711–723. DOI: 10.22138/2500-0918-2018-15-5-711-723 (In Russ)

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Authors
Victor I. Tsirkin
Vyatka State university
Dr. Sci. (Med.), Professor of Department of Biology and Methods of Biology
Russian Federation, 610000, Kirov, ul. Moskovskaja, 36
tsirkin@list.ru

Konstantin Yu. Anisimov
Ural State Medical University
Cand. Sci. (Med.), Assistant, Department of Obstetricsand Gynecology
Russian Federation, 620028, Yekaterinburg, ul. Repina, 3
kuanisimov@mail.ru

Elena G. Shushkanova
Vyatka State University, Institute of Biology and Biotechnology
Cand. Sci. (Biol.), Associate Professor, Department of Biology and Methods of Biology
Russian Federation, 610000, Kirov, ul. Moskovskaja, 36
el.s90@mail.ru

Anatoly G. Kolpakov
Vyatka State University, Institute of Biology and Biotechnology
4 th year student
Russian Federation, 610000, Kirov, ul. Moskovskaja, 36
kolpakov5465@gmail.com

Elena E. Dushina
Vyatka State University, Institute of Biology and Biotechnology
4 th year student
Russian Federation, 610000, Kirov, ul. Moskovskaja, 36
lenochkadushina@mail.ru

Elena N. Buskova
Vyatka State University, Institute of Biology and Biotechnology
Graduate student, Physiologist of the Department of Biology and Methods
of Teaching of Biology
Russian Federation, 610000, Kirov, 36 Moskovsky St.
elena_bushkova@mail.ru

Maria V. Bysheva
Vyatka State University, Institute of Biology and Biotechnology
Graduate student
Russian Federation, 610000, Kirov, 36 Moskovsky St.
mbysheva@mail.ru

Tatiana V. Cherepanova
Women's consultation No. 9
Russian Federation, 610001, Kirov, Nekrasov st, 6A
gb9@medkirov.ru