ANTITUMOR EFFECT OF EMF-LF (5 Hz) ON MELANOMA CELL CULTURE B16-F10
DOI: 10.22138/2500-0918-2025-22-1-28-42
I.Y. Maklakova¹,², O.E. Knysh¹, K.A. Gavrilova¹, D.S. Erlish¹,
D.Y. Grebnev¹,², E.D. Uskov³
¹Federal State Budgetary Educational Institution of Higher Education
«Ural State Medical University» of the Ministry of Health of the Russian Federation, Yekaterinburg, Russian Federation;
²Institute of Medical Cell Technologies, Yekaterinburg, Russian Federation;
³Federal State Autonomous Educational Institution of Higher Education
«Ural Federal University named after the first President of Russia B.N. Yeltsin»,
Yekaterinburg, Russian Federation
Abstract. Introduction. Melanoma ranks fifth in terms of prevalence among all types of STDs in the world, demonstrating a rapid increase in the incidence rate over the past 10 years. The trend towards an increase in the prevalence of this MND, its growing resistance to existing therapies and, as a consequence, an increase in melanoma mortality indicates the need to consider new therapeutic approaches, one of which is the use of low-frequency electromagnetic fields (LF-EMF). The ability of EMF to inhibit melanoma cell population growth has been previously demonstrated, but this work is the first to investigate the effect of EMF on a melanoma cell line using an extremely low frequency — 5 Hz. The aim of the study is to identify the effect of EMF 5 Hz on the functional state of the melanoma cell line B16-F10. Materials and methods. The state of the B16-F10 melanoma cell line was analyzed after EMF irradiation (frequency 5 Hz, current force 5 A — 20 A — 30 A, single-bipolar field) for 1, 2, 3 hours. A review of the current scientific literature on the described topic. Results. In the mode of operation of the EMF 2 generator at exposition of 2 hours – increase of necrotic cells by 100% (p<0,001). In mode 3, exposure for 3 hours – increase in cells in a state of apoptosis by 24% with a pronounced dose dependence (p<0.05). Discussion. This study has confirmed that exposure to EMF-LF (5 Hz) is a promising approach in melanoma. Presumably, the observed effect (induction of oncocell death) is realized due to a change in intracellular Ca²⁺ homeostasis, which requires further research.
Keywords: EMF, EMF-LF, low frequency electromagnetic fields, oncology, melanoma, B16-F10, apoptosis, necrosis, Ca, calcium
There is no conflict of interest.
Contact details of the corresponding author:
Oleg E. Knysh
knysh_oleg4@bk.ru
Received: 06.11.2024
For citation: Maklakova I.Y., Knysh O.E., Gavrilova K.A., Erlish D.S., Grebnev D.Y., Uskov E.D.
Antitumor effect of EMF-LF (5 Hz) on melanoma cell culture B16-F10. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2025, Vol. 22, no. 1, pp. 28–42. DOI: 10.22138/2500-0918-2025-22-1-28-42 (In Russ)
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Authors
Irina Y. Maklakova
Federal State Budgetary Educational Institution of Higher Education «Ural State Medical University» of the Ministry of Health of the Russian Federation
Prof., Dc. Sc. Med., Head of the Department of Normal Physiology
Yekaterinburg, Russian Federation
makliu@mail.ru
ORCID: 0000-0002-6895-7947
SPIN: 7335-7294
Oleg E. Knysh
Federal State Budgetary Educational Institution of Higher Education «Ural State Medical University» of the Ministry of Health of the Russian Federation
Student of the Faculty of Treatment and Prevention
Yekaterinburg, Russian Federation
knysh_oleg4@bk.ru
ORCID: 0009-0003-8070-6336
SPIN: 5422-6525
Ksenia A. Gavrilova
Federal State Budgetary Educational Institution of Higher Education «Ural State Medical University» of the Ministry of Health of the Russian Federation
Senior lecturer at the Department of Pathological Physiology
Yekaterinburg, Russian Federation
kberlyakova@mail.ru
ORCID: 0000-0002-4634-4066
SPIN: 7859-6210
Daria S. Erlish
Federal State Budgetary Educational Institution of Higher Education «Ural State Medical University» of the Ministry of Health of the Russian Federation
Student of the Faculty of Treatment and Prevention
derlish00@mail.ru
Yekaterinburg, Russian Federation
Dmitry Yu. Grebnev
Federal State Budgetary Educational Institution of Higher Education «Ural State Medical University»
Doctor of Medical Sciences, Associate Professor, Head of the Department of Pathological Physiology
Dr-grebnev77@mail.ru
ORCID: 0000-0002-5698-8404
SPIN: 5761-9324
Yekaterinburg, Russian Federation
Evgeny D. Uskov
Federal State Autonomous Educational Institution of Higher Education «Ural Federal University named after the first President of Russia B.N. Yeltsin»
Assistant of the Department of Technical Physics
uskov-evgeny@yandex.ru
Yekaterinburg, Russian Federation