Роль аллельного полиморфизма Т786С гена эндотелиальной NO-синтазы в формировании периферического тонуса сосудов
Bebyakova N.A., Feliksova O.M., Khromova A.V.
Northern State Medical University, Arkhangelsk, Russian Federation
Abstract. 168 male persons aged 18–22 years without complaints and heart problems were examined. It was investigated the association of this polymorphism with hemodynamics and vascular tone. We determined the basic parameters of hemodynamic and vascular tone. The study surveyed persons genotypes distribution of the studied polymorphism of NOS3 revealed that the ratio of normal homozygotes (TT), heterozygotes (TC) and pathological homozygotes (СС) — 42.3%, 46.4% and 11.3%, respectively. The most rare genotype is the СС, which according to several authors associated with the risk of arterial hypertension. It was found that allelic polymorphism T786C of endothelial NO-synthase gene not affect the mechanisms of systemic blood pressure, but participates in the formation of vascular tone. The higher peripheral vessels tone is a characteristic for persons with genotype CC. Persons with CC genotype characterized by more pronounced peripheral vasoconstriction, and they had a high index values of peripheral resistance after load (p<0.05) compared with other genotypes.
Keywords: peripheral vasoconstriction, endotheline-1, nitric oxide, T786C polymorphism in endothelial nitric oxide synthase
REFERENCES
1. Umans J. Nitric oxide in the regulation of blood flow and arterial pressure. J. Umans, R. Levi I I Ann. Rev. Physiol. — 1995. — Vol. 57. — P. 771 — 790
2. Evidence of association of the ecNOS gene polymorphism with plasma NO metabolite levels in humans. T. Tsukada, K. Yokoyam, T. Arai et al. II Biochem. Biophys. Res. Commun. — 1998. — Vol. 245. — P. 190—193.
3. Hingorani A. A simple molecular assay for the c1166 variant of the angiotensin II type 1 receptor gene. A. Hingorani, M. Brown. Biochem. Biophys. Res. Commun. — 1995. — Vol. 213. — P. 725 — 729.
4. Casas J.P. Endothelial nitricoxide synthase genotype and ischemic disease. Meta—analysis of 26 studies involving 23028 subjects. J.P. Casas L.E. Bautista S.E. Humphries, A.D. Hingorani II Circulation. — 2004. — Vol. 109. — P. 1359— 1365.
5. Zhang R. Functional analysis of the human endothelial nitric oxide synthase promoter. Spl and GATA factors are necessary for basal transcription in endothelial cells. R. Zhang, W. Min, W.C. Sessa. J. Biol. Chem. — 1995. — Vol. 270.-P. 15320—15326
6. T(786)C mutation in the 5'—flanking region of the endothelial nitric oxide synthase gene is associated with myocardial infarction, especially without coronary organic stenosis. M. Nakayama, H. Yasue, M. Yoshimura et al II Amer. J. Cardiology. — 2000. — Vol. 86, № 6. — P. 628—634.
7. The endothelial nitric oxide synthase (Glu298Asp and —786T>C) gene polymorphisms are associated with coronary instent restenosis. A.H. Gomma, M.A Elrayess, CJ Knight et al I I Eur. Heart J. — 2002. — Vol. 23. — P. 1955—1962.
8. Endothelial nitric oxide synthase gene is positivily assotiated with essential hypertension. Y. Miyamoto, Y. Saito, N. Kajiyama et al. Hypertension. —1998.—Vol. 32.—P. 3—8.
Authors
Bebyakova Natalya A., nbebyakova@mail.ru
Northern State Medical University
Doctor of biological sciences, Professor, Head of Department of medical biology and genetics
Russian Federation, 163000, Arkhangelsk, Troitsky prospekt, 51
Feliksova Olga M., felix2510@yandex.ru
Senior teacher of the Department of medical biology and genetics NSMU
Khromova Anna V., nadein@atknet.ru
Сandidate of medical sciences, Associate Professor of Department of medical biology and genetics