The Role of Apoptotic Factors in Damage to the Nervous Tissue and Restoration of its Functioning after Ischemic Stroke

DOI: 10.22138/2500-0918-2017-14-2-156-163

S. P. Sergeeva, L. M. Erofeeva, A. A. Savin,

P. F. Litvitskiy, L. V. Shishkina, V. V. Arkhipov

I.M. Sechenov First Moscow State Medical University, Moscow, Russian Federation;
Research Institute of Human Morphology, Moscow, Russian Federation;
A.I. Evdokimov Moscow State University of Medicine and Dentistry, Moscow, Russian Federation;
N.N. Burdenko Scientific Research Neurosurgery, Moscow, Russian Federation

Abstract. Objective: to identify the role of the apoptotic factors sFasL and sFas in damage to the nervous tissue and restore its functioning after an ischemic stroke. Materials and methods: the study included 155 ischemic stroke patients. The control group is healthy volunteers (n = 28). The concentration of sFas, sFasL was measured on days 1, 7 and 21 after IS by ELISA, the ratio of sFasL to sFas was calculated. After statistical processing of the data by the cluster analysis method, the patients who were in the hospital until 21 days were divided into groups depending on the IS acute period outcome — with a favorable one (the NIHSS score of 21 days was less than or equal to 5) (n = 36) and with an unfavorable outcome (score on the NIHSS scale for 21 days more than 5, and also died by 21 days) (n = 55). The autopsy samples of brain tissue of 9 people who died as a result of left middle cerebral artery IS from the group with an unfavorable outcome were studied. Samples of tissue were taken from 3 zones of the brain: 1 — adjacent to the foci of necrotic tissue, 2 — distant from the previous by 5–10 cm, 3 — the zone of the opposite hemisphere, symmetrical focus of ischemia. Samples were Nissl and hematoxylin-eosin stained. Proteins p53, NSE, GFAP were detected by an indirect immunoperoxidase immunohistochemical method. Samples were stained with Nissl, hematoxylin and eosin. Proteins p53, NSE, GFAP were detected by an indirect immunoperoxidase immunohistochemical method. Results. When pathomorphological examination, signs of neurons and gliocytes apoptosis in all investigated zones were observed. However, an increase in the ratio of sFasL to sFas by 21 days significantly correlates with the favorable outcome of the IS. Thus, the pleiotropic effect of the Fas system and the clinical significance ones in the development of IS have been demonstrated.

Keywords: stroke, Fas, apoptosis, neuroplasticity

Received 19.04.2017

For citation:
Sergeeva S.P., Erofeeva L.M., Savin A.A., Litvitskiy P.F., Shishkina L.V., Arkhipov V.V. The role of apoptotic factors in damage to the nervous tissue and restoration of its functioning after ischemic stroke. Vestn. Ural. Med. Akad. Nauki = Jour. Ural Med. Acad. Science. 2017, no. 2, pp. 156–163. DOI: 10.22138/2500-0918-2017-14-2-156-163 (In Russ.)

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Authors
Svetlana P. Sergeeva
I. M. Sechenov First Moscow State Medical University
Cand. Sci. (Med.), Associate Professor of the Department for Pathophysiology
Str. Trubetskaya, 8-2, Moscow, 119991, Russian Federation
svetlanapalna@mail.ru

Lyudmila M. Erofeeva
Institute of Human Morphology
Dr. Sci.(Bio), Professor
Str. Tsyurupy, 3, Moscow, Russian Federation, 117418
gystology@mail.ru

Alexey A. Savin
A.I. Evdokimov Moscow State University of Medicine and Dentistry, Department for Neurology
Dr. Sci.(Med), Professor
Str. Delegatskaya, 20/1, Moscow, 127473, Russian Federation
lasavin@mail.ru

Peter F. Litvitskiy
I.M. Sechenov First Moscow State Medical University
Corr.-Member Russian Academy of Sciences, Dr. Sci.(Med), Professor, Head of the Department for Pathophysiology
Str. Trubetskaya, 8-2, Moscow, 119991, Russian Federation
litvicki@mma.ru

Lyudmila V. Shishkina
N.N. Burdenko Scientific Research Neurosurgery
Dr. Sci.(Med), Professor, Head of Department for Pathological
Str. 4th Tverskaya-Yamskaya, 16, Moscow, 125047, Russian Federation
lshishkina@nsi.ru

Vladimir V. Arkhipov
I.M. Sechenov First Moscow State Medical University
Dr. Sci.(Med), Professor, Department for Neurology
Str. Trubetskaya, 8-2, Moscow, 119991, Russian Federation
arkhipov2005@rambler.ru

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