SCREENING OF FUNCTIONAL, BIOCHEMICAL AND CELLULAR HEMATOLOGIC PARAMETERS OF THE HUMAN ORGANISM AS MARKERS OF THE PROCESS OF HUMAN AGING

DOI: 10.22138/2500-0918-2018-15-5-691-703

I.V. Gavrilov, V.N. Meshchaninov, D.L. Shcherbakov, V.A. Lukash, E.M. Varlashov

Institute Medical Cell Technologies, Yekaterinburg, Russian Federation;
Ural State Medical University, Yekaterinburg, Russian Federation

Abstract. Actuality. Modern gene diagnostics is functional and metabolic basis of geroprophylaxis. To characterize the rate of ageing, reliable indicators or biomarkers are needed to accurately characterize the complex mechanism of ageing. The search and evaluation of aging biomarkers should be comprehensive, and include experimental, clinical, «omix» and integrative approaches. One of the methods that can precede these approaches at the screening stage is pair and multiple correlation analysis, which characterizes the statistical relationship between the potential biomarkers of aging and calendar age. Objective. To be revealed by correlation analysis of the change in the power and number of correlations of potential markers of aging in terms of physiological and accelerated aging and its correction for the detection of new potential markers of the aging process and to verify their adequacy. Results. With aging, there was a decrease in accommodation of the lens of the eye, hearing acuity, there was a decrease in the indicator of static balancing, lung capacity and indicators of the Wexler test. These psycho-physiological parameters were highly correlated with calendar age. With increasing age, there was a decrease in the peripheral blood platelet count, significantly reduced the content of stem hematopoietic and progenitor cells in combination with the appearance of a significant inverse (negative) correlation with calendar age, and there was a strong negative feedback between calendar age and the content of the marker CD34+. Correlation approach to identify significant markers of functional, laboratory and psychological indicators of aging in healthy patients, in terms of polymorbid pathology and its correction, has revealed the most important basic of personalized markers of aging to create on their basis effective gerodiagnostics technology that was verified corrective geroprophylaxis events that led to the decrease of the biological age in all age groups of patients of both sexes. Materials and methods. The work was performed on the basis of data of voluntarily examined 5296 patients (3395 males and 1901 females) aged 20-90 years, divided by sex, age, health status, practically healthy patients, patients with polymorbid pathology, as well as a group of patients with polymorbid pathology, receiving various types of basic and geroprotective therapy. The age dynamics of biochemical parameters of peripheral blood, including lipid peroxidation and antioxidant activity (POL/AOA), cellular-hematological and functional-psychological indicators in physiological aging, accelerated polymorbid pathology of aging, in the treatment of polymorbid pathology were studied. The presence of the relationship of indicators with the calendar age was determined by calculating the Pearson pair correlation coefficient ® and its significance by parametric criteria. Summary. 1. In patients among the functional-psychological and cellular-hematological parameters, found those in which the strength and (or) the number of correlations with calendar age is significantly higher (indicators of lung capacity, accommodation of the lens of the eye, hearing acuity, static balancing, platelet content, stem hematopoietic and progenitor cells in peripheral blood) than the standardized («routine») biochemical parameters. 2. Positive results reduce biotrust in patients after correction geroprophylaxis events and treatment polymorbidity pathology under the condition of measurement of biological age calculated by using the identified markers, confirm the concept of the search for markers of aging using correlation analysis. 3. The correlation approach to the identification of significant laboratory and functional-psychological indicators reflecting the aging process in healthy patients, in the conditions of polymorbid pathology and its correction, based on the priority of significant differences in arithmetic mean values and absolute values of correlation coefficients, as well as their number, allowed us to find the most significant basic personalized markers of aging for male, female, young and middle, elderly and senile patients, that is confirmed by the results of the evaluation of the effectiveness of our proposed gerodiagnostics and therapeutic technologies.

Кeyword: screening, biomarkers, aging, diagnostic indicators

There is no conflict of interest.

Contact information of the author responsible for correspondence:
Victor N. Meshchaninov
mv-02@yandex.ru

Received 28.11.2018

For citation:
Gavrilov I.V., Meshchaninov V.N., Shcherbakov D.L., Lukash V.A., Varlashov E.M. Screening of functional, biochemical and cellular hematologic parameters of the human organism as markers of the process of human aging. Vestn.Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2018, Vol. 15, no. 5, pp. 691–703. DOI: 10.22138/2500-0918-2018-15-5-691-703 (In Russ)

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Authors
Iliya V. Gavrilov
Cand.Sci. (Bio.)
given18@yandex.ru

Victor N. Meshchaninov
Professore, Dr. Sci. (Med.)
mv-02@yandex.ru

Denis L. Shcherbakov
Cand.Sci. (Bio.)
cdcom2@yandex.ru

Vyacheslav A. Lucash
Cand.Sci. (Bio.)
kafedra.biohimii@yandex.ru

Varlashov Evgenii M.
Student
varlash@mail.ru

Institute for Medical Cell Technologies;
22a, Karla Marksa st., Yekaterinburg, Russian Federation, 620026
Ural State Medical University