COMPARATIVE EVALUATION OF PRODUCTS OF OXIDATIVE MODIFICATION OF LIPIDS AND PROTEINS IN TISSUES OF EXUDATIVE LESIONS OF REINKE’S SPACE
DOI: 10.22138/2500-0918-2022-19-4-379-385
M. A. Kovalev¹, E. V. Davydova², A. V. Zurochka³,⁴
¹ Branch No. 3, Federal State Budgetary Institution ‘‘3, National Medical Research Center
for High Medical Technologies named after A. A. Vishnevsky’’
of the Ministry of Defense of the Russian Federation, Odintsovo, Russian Federation;
² South Ural State Medical University of the Ministry of Health of Russia,
Chelyabinsk, Russian Federation;
³ Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences, Yekaterinburg, Russian Federation;
⁴ South Ural State University (National Research University), Chelyabinsk, Russian Federation
Abstrsct. Exudative lesions of Reinke’s space (EPPR) include polyps, vocal cord nodules, Reinke’s edema itself and rank high in the ranking of benign productive non-inflammatory pathology of the larynx. Occupational phonotrauma of the vocal fold mucosa, often in combination with exposure to toxic factors, contributes to a change in tissue and immune homeostasis with subsequent remodeling, elements of chronic inflammation, hypoxic-ischemic changes and the development of oxidative cellular stress. The aim of the work was a comparative assessment of the content of products of oxidative modification of lipids and proteins in biopsy specimens of neoplasms of the vocal folds. Materials and methods: products of oxidative modification of lipids in biopsy samples were determined using the extraction-spectrophotometric method in the heptane and isopropanol phases of the lipid extract, the concentration of modified proteins by reaction with 2,4-dinitrophenylhydrazine. Statistical processing of the material was carried out using the Statistica 10.0 for Windows. Results: The data obtained indicate the predominance of accumulation in the tissues of «soft» EPPR (myxoid-type polyps and Reinke’s edema) of products of oxidative modification of lipids and proteins, in comparison with «sweaty» ones (agnomatous-type polyps and vocal nodules), which reflects the severity of hypoxic- ischemic changes.
Keywords: exudative lesions of Reinke’s space, oxidative modification of lipids and proteins
There is no conflict of interest.
Contact details of the corresponding author:
Evgeniya V. Davydova
davidova-ev.med@yandex.ru
Received 13.10.2022
For citation:
Kovalev M.A., Davydova E.V., Zurochka A.V. Comparative evaluation of products of oxidative modification of lipids and proteins in tissues of exudative lesions of reinke’s space. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2022, Vol. 19, no. 4, pp. 379–385. DOI: 10.22138/2500-0918-2022-19-4-379-385 (In Russ)
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Authors
Mikhail A. Kovalev
Branch No. 3, Federal State Budgetary Institution “3, National Medical Research Center for High Medical Technologies named after V.I. A.A. Vishnevsky» of the Ministry of Defense of the Russian Federation
Head of the Department of Otorhinolaryngology,
1 st. Marshal Biryuzov Odintsovo Moscow region Russian Federation 143000
lor.kovalev@mail.ru
ORCID: 0000-0002-3084-2765
Evgeniya V. Davydova
South Ural State Medical University
MD, Head of the Rehabilitation Department,
70 Vorovskyi st. Chelyabinsk Russian Federation 454048
davidova-ev.med@yandex.ru
ORCID: 0000-0001-6392-8971
Alexander V. Zurochka
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences
Leading Researcher, Laboratory of Inflammation Immunology,
South Ural State University (National Research University)
МD, Professor, Professor of the Department of Food and Biotechnology, Head of the Laboratory of Immunobiotechnology
106 st. Pervomaiskaya Yekaterinburg Russian Federation 620049
av_zurochka@mail.ru
ORСID 0000-0003-4371-4161